Mechanisms deciphering enzyme

Another difficulty which has faced the x-ray crystallographic approach to deciphering enzyme mechanisms is the inability to examine enzyme-substrate complexes. To circumvent this problem, complexes with inhibitors, products, or pseudosubstrates have been employed. In the case of carboxypeptidase A, mechanistic deductions have been based on results obtained using the very poor substrate glycyl-L-tyrosine (60). 

Deciphering Enzyme Mechanisms by Studying Promiscuous Functions 61... 

The development of cell free systems has played an important role in attempts to decipher the mechanism of peptide antibiotic synthesis ". Most workers agree that this type of synthesis occurs in the complete absence of polynucleotides and that the amino acid sequence is determined by enzyme specificity and organisation in a multi-enzyme complex . The tyrocidines are produced in Bacillus brevis by an enzyme system which has been resolved into three complimentary fractions by Sephadex G-200 filtration a light and an intermediate component (molecular weight 100000 and 230000) which activate L-phenylalanine and L-proline respectively and a heavy fraction (460 000) which activates the remaining eight amino acids including L-phenylalanine. Each... 

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