Mechanical agitation enzymes

Most of the known microorganisms to be active for BDS (by the date that patent [408] was introduced), were considered in this invention. Furthermore, their mutational or engineered derivatives, enzymes, cell-free extracts, recombinant enzymes, recombinant DNA, plasmids, vectors, and fragments were also incorporated in the intellectual property document. The mentioned operating conditions regard ambient temperature, mechanical agitation and a 1 9 biocatalyst/petroleum ratio. 

Reactions of cell growth or those using immobilized enzymes are instances of gas-liquid-solid reactions. In principle, accordingly, any of the types of reactors described in Section 8.3 could be employed as fermentors. Mostly, however, mechanically agitated tanks are the type adopted. Aeration supplies additional agitation as well as metabolic need, and moreover sweeps away C02 and noxious byproducts. 

Silva C, Araujo R, Casal M et al (2007) Influence of mechanical agitation on cutinases and protease activity towards polyamide substrates. Enzyme Microb Technol 40 1678-1685... 

Radhakrishnaiah P, Menge X, Huang G, Buschle-Diller G and Walsh W K, Mechanical agitation of cotton fabrics during enzyme treatment and its effect on tactUe prop>erties . Textile Res. J., 1999, 69(10), 708-13. 

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