Measurement of hypericin and hyperforin in human plasma using HPLC

4 Measurement of hypericin and hyperforin in human plasma using HPLC 

Hyperforin concentration in human plasma can be measured using HPLC with UV detection at 287 nm. A Luna Cig column (150 x 4.6 mm i.d., particle size 3 pm, Phenom-enex) was used and the mobile phase was prepared by adding a methanol-acetonitrile organic phase (3 2 v/v) to water so that the final composition of the organic phase was 92% and the aqueous phase 8%. Finally, 2 mL of formic acid and 2 mL of triethylamine were added to 1000 mL of mobile phase and the pH of the final mobile phase was 3.2. Hyperforin-containing or spiked plasma was mixed with acetonitrile and finally hyperforin was extracted using a solid-phase extraction colunrn. Benzo[fc]fluoranthene was used as the internal standard. The 

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