MDA-MB-231 breast cancer cell line

Meegan et al. reported the synthesis of a number of [3-lactams and these were evaluated in a series of in vitro assays, which determined their antiproliferative activity in MCF-7 and MDA-MB-231 breast cancer cell lines and also their affinity for the oestrogen receptor (Fig. 6). The cytotoxicity of the [3-lactams was determined in the LDH assay to establish that the antiproliferative effects observed were due to cytostasis rather than cellular necrosis. Most of the compounds demons-treated low cytotoxicity, indicating that their action is cytostatic rather than cytotoxic. Cytotoxicity values considerably below that obtained for tamoxifen (13.4%, 10 pM) were observed with a most potent compound (3%, 10 pM in MCF-7 cell... 

Compound MDA-MB-231, Breast Cancer Cell Line. ICjq, xM References... 

Ferrocenyl catechols 8 and 9 also exhibited interesting cytotoxic activities. On MDA-MB-231 breast cancer cell lines, the catechol compounds displayed a similar or greater antiproliferative potency (IC50 values ranging from 0.48 to 1.21 p,M) than their corresponding phenolic analogs (0.57-12.7 p.M) in complete agreement with their easiest oxidation [28]. 

Interestingly, the cytotoxic activity of ferrocifen compounds is not restricted to phenolic substituents. For instance, the substitution of the hydroxyl group by another protic and oxidizable functionality such as aniline or acetanilide (compounds 10 and 11, respectively) also provides strong cytotoxic action against hormone-dependent MCF-7 and hormone-independent MDA-MB-231 breast cancer cell lines [30]. 

The RXR-RXR antagonist LDG100754 and RAR-selective TTNPB activated RXR-RAR heterodimers on DR-2 and DR-5 RAREs, whereas LDG100268 did not however, only TTNPB dissociated the corepressor SMRT from the RXR-RAR complex [36]. The synergy between RXR-selective retinoids and RAR-selective retinoids was ably demonstrated by Wu et al. [105]. RXR-selective SRI 1345 at 1.0 pM, which only inhibited MDA-MB-231 breast cancer cell growth by less than 5%, enhanced the inhibitory activity of RAR-selective SRI 1365 from below 5% to 55%. In the ZR-75-1 human breast cancer cell line, inhibition by SRI 1365 rose from 40% to 70%, after addition of SRI 1345, which alone was inactive. 

Anticancer EGb-761 Dose-dependent decreases in xenograft growth of both MDA-MB-231 breast cancer and U-87 glioma cell lines in nude mice suppress proliferation and increase cytotoxicity in HepG2 and Hep3B cells [27, 28]... 

An tmusual association between a sesquiterpene and a purine was isolated from Suhergorgia suherosa, a species of Chinese origin. The sesquiterpenic part of this atypical alkaloid has the carbon skeleton of quadrane (see below), and this derivative is slightly cytotoxic for the cell line MDA-MB-231 (breast cancer). 

Fig. 10.19 Lack of toxic effects of CM fullerene on breast epithelial cells. Cm does not inhibit cell proliferation. MCF 10A and (A) MDA MB 231 (B) breast cancer cell lines were cultured either in the absence or presence of methanol C60 and cell proliferation was assayed by crystal violet staining. Control, no C 1 Opg Cm, A 50 pg Cm, X 250 pg CM. (C). MDA MB 231 cells were simultaneously stained with calcein and ethidium using a live-dead assay kit. Lack of red-colored cells and the presence of cells stained in green indicate the lack of toxicity (D). MDA MB 231 cells were either untreated (open box) cultured with varying amounts 10 (gray ), 50 (patterned ) and lOOpg (filled ) of C60 for 48 h and analyzed for cell cycle progression by flow cytometry (Levi et al., 2006) (See Color Plates)...

Gallagher, S.M., Castorino, J.J., Wang, D. and Philp, N.J. (2007) Monocarboxylate transporter 4 regulates maturation and trafficking of CD147 to the plasma membrane in the metastatic breast cancer cell line MDA-MB-231. Cancer Research, 67 (9), 4182—4189. 

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