MCF7 cell proliferation assay

Breinholt, V. and J.C. Larsen. 1998. Detection of weak estrogenic flavanoids using a recombinant yeast strain and a modified MCF7 cell proliferation assay. Chem. Res. Toxicol. 11 622-629. 

AG and APG were assayed in the E-Screen test for estrogenicity but were ineffective in inducing MCF7 cell proliferation. Table 7.3.3 shows the results of the proliferation test. 

Desaulniers D, Leingartner K, Zacharewski T, Foster WG (1998) Optimization of an MCF7-E3 cell proliferation assay and effects of environmental pollutants and industrial chemicals. Toxicol In Vitro 12(4) 409 422... 

In the E-Screen bioassay, LAS was not effective in promoting cell proliferation (Table 7.3.3). This compound was tested at concentrations of up to 100 pM with no evidence of cellular toxicity. The antiestrogenic effect of this compound was also measured but all samples tested were negative. Because it has been suggested that surfactants of the alkylbenzene sulfonate type are readily degradable and transformed into sulfophenyl carboxylates or SPCs, an important number of SPCs were assayed in the E-Screen test. These SPCs did not induce cell proliferation of MCF7 cells. 

Linear alcohols were studied using MCF7 cells in the proliferation assay and in the expression of estrogen-dependent genes (Table 7.3.4). The AS C12 i4, its ethoxylated derivative (ALFONIC 1214) and linear alcohols from nonanol to dodecanol increase cell proliferation in the E-Screen assay. This effect was seen at 10-100 xM concentrations and,... 

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