Maximum Sample Injection Volume for a Specific Column

8 Maximum Sample Injection. Volume for a Specific Column 

Details of the injection of a sample onto a column will inevitably contribute an additional source of peak broadening that will increase as the injected volume is increased. It would be useful to be able to estimate the 

It is also necessary to ensure that the sample extract to be injected on column uses a solvent that is either the LC mobile phase itself or a solvent of lower elution strength (Section 4.4.2a). 

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