Matrix picolinic acid

Tang, K. Taranenko, N.I. Allman, S.L. Chen, C.H. Chang, L.Y. Jacobson, K.B. Picolinic Acid As a Matrix for Laser-MS of Nucleic Acids and Proteins. Rapid Commun. Mass Spectrom. 1994, 8, 673-677. 

Wu, K.J. Steding, A. Becker, C.H. Matrix-Assisted Laser Desorption-TOF-MS of Oligonucleotides Using 3-Hydroxy-picolinic Acid As an Ultraviolet-Sensitive Matrix. Rapid Commun. Mass Spectrom. 1993, 7,142-146. 

IPC is also effective for pharmacology related to brain disease. It was used to analyze picolinic acid and related compounds [90], neurotoxins associated with paralytic shellfish poisoning [91], a drug candidate for treating Alzheimer s disease [92], and nicergoline, clinically used for improving brain metabolism [93]. Quaternary ammonium anticholinergics were determined in whole blood and the matrix effect was taken into account [94]. 

K. Tang, N. I. Tarmenko, S. L. Allman, C. H. Chen, L. Y. Ch ang, and K. B. lacobson, Picolinic acid as a matrix for laser mass spectrometry of nucleic acids and proteins. Rapid Commun. Mass Spectrom. 8, CTi-611 (1994). 

Figure 4 Representation of the structures of some commonly used matrices for ultraviolet matrix-assisted-laser desorption/ionization (MALDI). These matrices are used with nitrogen lasers emitting at 337 nm. a-Cyano-4-hydroxycinnamic acid is widely used for the analysis of peptides the picolinic acid derivatives are more suitable for oligonucleotides.

Figure 20 DE-MALDI-TOF spectrum of a mixture of Sanger dideoxy DNA sequencing reactions containing about 0.1 pmol of each termination fragment generated by primer extension on a 50-base-pair template. The matrix used was a mixture of of 3-hydroxypicolinic acid and picolinic acid including diammonium citrate to complex metal ions (prepared according to Wu et al. [98]). The four panels correspond to termination with (A) ddA. (B) ddC, (C) ddG, and (D) DDT. (From Ref. 82.)...

FIGURE I 1.22 Delayed -extraction MALDI-TOF mass spectra of the I I-oligonucleotide d(CACACGCCAGT) using (a) a pulsed nitrogen laser and 3-HPA as the matrix and (b) a frequency-quadrupled Nd YAG laser and picolinic acid as the matrix, (c) Assignment of the fragment ion peaks. (Reprinted with permission from reference 23). 

Nicotinic add (NA) was the first organic compound to be successfully employed as a matrix in UV-MALDI of peptides and proteins [12-14]. Ever since, better matrices have been sought, the following now being widespread in use (Fig. 11.8) picolinic acid (PA) [67], 3-hydroxypicolinic acid (HPA) [68], 3-aminopicolinic add (3-APA) [69], and 6-aza-2-thiothymine (ATT) [70,71] for oligonucleotides... 

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