Cells Matrigel-induced angiogenesis

To characterize the antitumor and/or antimetastatic activities, the effects of carp oil and oleic acid on DNA synthesis in LLC cells, adherence of LLC cells to human adult dermal microvascular endothelial cells (HMVEC), and Matrigel-induced angiogenesis (in vitro and in vivo) were tested. Carp oil inhibited DNA synthesis in LLC cells at 100 and 1000 pg/mL, but not at 0.1 to 10 pg/mL Table (10) . Oleic acid inhibited DNA synthesis in LLC cells at 1000 pM Table (11) . Carp oil inhibited the Matrigel-induced tube-like network formation of HMVEC at 1000 pg/mL, but not at 0.1 to 100 pg/mL Table (10) . 

Apart from fibrin, collagen, and alginate, other natural polymers that have shown promising results in vivo are chitosan, Matrigel, hyaluronic acid, and decellularized ECM [14]. For example, Fujita et al. showed efficient angiogenesis and collateral flow induced by FGF-2 loaded in chitosan HGs. Notably, the chitosan HG allowed an extended FGF-2 delivery for more than 1 month [76]. Moreover, chitosan HGs combined with stem cells also resulted in increased angiogenesis, reduced adverse remodeling and preserved heart function compared to free cells or nonloaded chitosan HG administration [77]. 

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