Dual-channel mass spectrometer

These three examples illustrate technology developments over time (dual-channel detector, diode array detector, mass spectrometer). Note that while the overall methodology is very similar (methanolic extracts, methanol-based, acidified solvents used for HPLC, detection of eluted compounds), the exact conditions for successful separation need to be defined for each system. 

UV-visible spectra were obtained using a Cary 118C spectrophotometer. Insertion probe mass spectra were obtained with a Dupont 21-49IB mass spectrometer, the FAB spectra with a VG MM-ZAB instrument, and the FD spectra with a JEOL DX-300 mass spectrometer. High pressure liquid chromatography measurements were made with a Waters instrument fitted with dual channel (405 and 546 nm) optical detection and using C18 analytical and semipreparative columns. 

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