Mass Spec Analytical

R Sleeman and J F Carter, Mass Spec Analytical Ltd., Bristol, UK... 

Mass Spec Analytical (MSA) Ltd. also conducted research on the analysis of Ecstasy tablets from different batches. The analysis and comparison of Ecstasy tablets using IRMS has subsequently been used in a number of police cases [88,157]. 

Typically, the characterization of supramolecular systems requires the application of a whole set of analytical techniques. Without going into details, the capsular structure of the tennis ball 1-1 has been confirmed by X-ray crystallography, vapor phase osmometry (VPO), and NMR experiments.1111 The encapsulation of guests can be routinely analyzed by NMR experiments. Recently, a mass spec-trometric method was developed that not only... 

Mass spectrometry can play a key role in the identification of the constituents of feedstocks and products (Aczel, 1989). The principal advantages of mass spec-trometric methods are (1) high reproducibility of quantitative analyses, (2) the potential for obtaining detailed data on the individual components and/or carbon number homologs in complex mixmres, and (3) the minimal sample size required for analysis. The ability of mass spectrometry to identify individual components in complex mixtures is unmatched by any modem analytical technique perhaps the exception is gas chromatography. 

Jones JJ, Kidwell H, Games DE. 2003. Application of atmospheric pressure chemical ionization mass spectrometry in the analysis of barbiturates by high-speed analytical countercurrent chromatography. Rapid Commun Mass Spec-trom 17 1565. 

As discussed in Section 2.2.3, proteomic investigations require powerful analytical techniques such as those continuing to be developed using mass spectrometry. As Aebersold and Mann declare in a 2003 review of mass spec-... 

Figure 4.4. Schematic of an orthogonal Q-TOF mass spectrometer. In this example, an ion beam is produced by electrospray ionization (ESI). The solution introduced to the mass spec-trometer-ESI source may be an effluent from a liquid chromatography column or simply an infusion solution of an analyte.

Roche S et al (2009) Development of a high-performance liquid chromatographic-mass spec-trometric method for the determination of cellular levels of the tyrosine kinase inhibitors lapatinib and dasatinib. J Chromatogr B Analyt Technol Biomed Life Sci 877 3982-3990... 

Chi C, Liang L, Padovani P et al. (2003) Development and validation of a liquid chromatography-mass spectrometric method for the determination of DPC 423, an antithrombotic agent, in rat and dog plasma. J Chromatogr B Analyt Technol Biomed Life Sci 783( 1) 163—172 Zimmer D, Muschalek V, Muller C (2000) Determination of met-rifonate enantiomers in blood and brain samples using liquid chromatography on a chiral stationary phase coupled to tandem mass spectrometry. Rapid Commun Mass Spec-trom 14(15) 1425-1432... 

Another advantage of the use of tandem mass spectrometry is the ability to isolate a particular ion such as the molecular ion of the analyte of interest during the first mass spec-... 

For further analytical investigations 0.2 mg polymer samples were removed from electrodes and pyrolysed in a Jeol pyrolyser unit, Model 727. Pyrolyses were carried out in a helium atmosphere at a temperature of 400° C for 30 sec. The volatile pyrolysis products were fed directly to the injection part of a Jeol gas chromatograph, Model JGC 1100, equipped with a flame ionization detector the apparatus incorporated a 2.5 m X 2 mm stainless steel separation column filled with OV 101 10 % supported on Varaport 80/100 mesh. The volatile pyrolysis products, isolated by gas chromatography, were analysed using a LKB mass spectrometer,Model 2091, equipped with a PDP11 computer. Mass spec-trometric analyses were carried out with an electron beam energy of 70 eV. 

Quadrupole mass spectrometers have relatively low resolution and have some problems with interferences. Two elements with the same atomic weight, e.g., CaO and Fe, both with a mass of 56, cannot be distinguished. Quadrupole mass specs have advantages, however, of being relatively simple and small these instruments, for example, can be placed on board spacecraft or combined with other analytical instruments such as chromatographs for the identification of molecules, discussed below. 

When compared to the analysis of hybridization events by detecting labels -even on arrays, the DNA MassARRAY approach differs significantly. The MassEXTEND assay is designed to give only the relevant information. The mass spec-trometric approach enables a direct analyte detection with 100% specificity and needs no redundancy. This accuracy and efficacy is combined with sample miniaturization, bioinformatics and chip-based technologies for parallel processing of numerous samples. 

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