Mass screening constituents

J.-L. Wolfender, S. Rodriguez and K. Hostettmann, Liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy for the screening of plant constituents. J. Chromatogr.A 794 (1998) 299-316. 

J.-L. Wolfender, S. Rodriguez, K. Hostettmann, LC coupled to MS and NMR spectroscopy for the screening ofplant constituents, J. Chromatogr. A, 794 (1998) 299. D.M. Drexler, P.R. Tiller, S.M. Wilbert, F.W. Bramble, J.C. Schwartz, Automated identification of isotopically labeled pesticides and metabolites by intelligent real time LC-MS-MS using a bench-top ion trap MS, Rapid Commun. Mass Spectrom., 12 (1998) 1501. 

The immobilized constituent can be a drug receptor protein containing a binding site that emulates the site of pharmacological action. As described in Chapter 7, combinatorial libraries can be screened by allowing drug candidates to bind to the site in a chromatographic flow situation. These candidates are then removed from the site by denaturization and are subsequently identified by a downstream mass-spectrometry experiment [39]. 

Wolfender J-L, RodriguezS, Hostettmann K (1998) Liquid Chromatography Coupled to Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy for the Screening of Plant Constituents. J Chromatography A 794 299... 

Under the conditions used, only low molecular mass constituents are observable, the signals from proteins and other large molecules are not resolved. Applications include screening and monitoring for metabolic diseases, the study of biochemical mechanisms associated with disease processes, and the identification of drug metabolites. 

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