Markers myeloperoxidase

Heinecke JW, Li W, Mueller DM, Bohrer A, Turk J (1994) Cholesterol Chlorohydrin Synthesis by the Myeloperoxidase-Hydrogen Peroxide-Chloride System Potential Markers for Lipoproteins Oxidatively Damaged by Phagocytes. Biochemistry 33 10127... 

Hazen SL, Heinecke JW (1997) 3-Chlorotyrosine, a Specific Marker of Myeloperoxidase-Catalyzed Oxidation, Is Markedly Elevated in Low Density Lipoprotein Isolated from Human Atherosclerotic Intima. J Clin Invest 99 2075... 

Buss IH, Senthilmohan R, Darlow BA, MogridgeN, Kettle AJ, Winterboum CC (2003) 3-Chlorotyrosine as a Marker of Protein Damage by Myeloperoxidase in Tracheal Aspirates from Preterm Infants Association with Adverse Respiratory Outcome. Pediatr Res 53 455... 

Instead of myeloperoxidase as a marker enzyme, 3-glucuronidase, or elastase can be used with similar success. Myeloperoxidase measurement gives the fastest results, which might be important for doing additional HPLC the same day. 

H13. Hazen, S. L., and Heinecke, J. W., 3-Chlorotyrosine, a specific marker of myeloperoxidase-catalyzed oxidation, is markedly elevated in low density lipoprotein isolated from human atherosclerotic intima. J. Clin. Invest. 99, 2075—2081 (1997). 

A solution of 3% acetic acid was instilled intracolonically into mice. After 24 hours, the mice were killed and colonic tissue samples were collected. Tissue samples were assessed biochemically for the neutrophil marker enzyme myeloperoxidase (MPO). Mn(ii) complexes were given intra-colonically 30 minutes prior to acetic acid. 

Heinecke, J. W., W. Li, D. M. Mueller, A. Bohrer, and J. Turk. 1994. Cholesterol chlorohydrin synthesis by the myeloperoxidase-hydrogen peroxide-chloride system Potential markers for lipoproteins oxidatively damaged by phagocytes. 33(33) 10127-36. 

Myeloperoxidase found within atherosclerotic lesions is also thought to provide a link between inflammation and cardiovascular disease. This enzyme is recognized as a major catalyst of lipid oxidation at sites of inflanunation by the formation of nitric oxide derived oxidants, proatherogenic halogenated fatty aldehydes and unsaturated lysophospholipids. The oxidants produced by myeloperoxidase are considered as part of its normal function in host defense. Because the levels of this enzyme are significantly increased in persons afflicted with cardiovascular disease, it is emerging as an important marker of oxidant stress in assessing cardiovascular risk. 

Brennan, M-L. and Hazen, S.L. Emerging role of myeloperoxidase and oxidant stress markers in cardiovascular risk assessment. Current Opinion in Lipidology 14, 353-359 (2003). 

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