Marker enzymes catalase

Most EIA use HRP as the marker enzyme. The activity of HRP can be measured photometrically as well as electrochemically. Using the catalase activity of liver tissue, Mascini and Palleschi (1983b) developed a tissue-based electrode for the measurement of hydrogen peroxide and combined the sensor with commercial test kits for digoxin and insulin. The HRP-labeled hormones of the test kit compete with antigen in the sample in a test tube. The bound HRP activity is inversely proportional to the concentration of insulin and digoxine, respectively. 

Fig. 2. Subcellular distribution of a-oxidation activity. Fractions were isolated by differential centrifugation of cucumber homogenate. Recoveries were between 71 and 84 % for the marker enzymes (A) malate dehydrogenase, (B) succinate cyt c reductase, (C) catalase, and 94 % for the (D) a-oxidation activity.

Catalase is a marker peroxisomal enzyme. The degree of inhibition of all three catalases assayed was very similar among them. This effect can be used for quality control as the compound loses its selective cytotoxicity, the inhibiting capacity also diminishes... 

ASP Acanthopanax senticosus scavenging ability in-vitro. Reduction of the levels of lipid peroxidation markers and increcise of the level of enzymic antioxidants (SOD) and catalase (CAT) activities in-vivo [120]... 

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