Mapping single molecule

Goulet, P. J. G., Pieczonka, N. P. W., and Aroca, R. F. (2005). Mapping single-molecule SERRS from Langmuir-Blodgett monolayers on nanostructured silver island films. Journal of Raman Spectroscopy 36 574-580. 

Thus electrostatic potential maps can give an exag gerated picture of the charge distribution when the entire palette is used In most cases that won t mat ter to us inasmuch as we are mostly concerned with the distribution within a single molecule In those few cases where we want to compare trends in a se ries of molecules we II use a common scale and will point that out... 

Shelby, J.P., Chiu, D.T., Mapping fast flows over micrometer-length scales using flow-tagging velocimetry and single-molecule detection. Anal. Chem. 2003, 75, 1387-1392. 

J. Veerman, M. Garcia-Parajo, L. Kuipers and N. van Hulst, Single molecule mapping of the optical field distribution of probes for near-field microscopy, J. Microsc. 194, 477 (1999). 

Fig. 2.4. (A) Sketch of the cryostat insert for single-molecule spectroscopy by fluorescence excitation. The focus of lens L is placed in the sample S by the magnet/coil pair M, C. (B) Scan over the inhomogeneous line (a) with a 2 GHz region expanded (b) to show isolated single-molecule absorption profiles. (C) Three-dimensional pseudo-image of single molecules of pentacene in p-terphenyl. The measured fluorescence signal (z-axis) is shown over a range of 300 MHz in excitation frequency (horizontal axis, center = 592.544 nm) and 40 pm in spatial position (axis into the page). (D) Rotation of the data in (c) to show that in the spatial domain, the single molecule maps out the shape of the laser focal spot. Bar, 5 pm. For details, see [33]...

Mapping Transcription Factors on Extended DNA A Single Molecule Approach... 

Finally, it is worthwhile imagining the possible ultimate resolution limits. So, if we had the perfect switchable marker emitting a bunch of m 3> 10 photons, what would we obtain for the stochastic single molecule switching mode We would obtain the (average) position of a molecule with a precision of a fraction of a nanometer. While this information would be invaluable for mapping the sample, it would not tell us much about the molecule itself. 

High-throughput single-molecule force spectroscopy has the potential to map binding sites of ligands at the single residue level and determine their binding constant while the respective membrane bound receptor is in its native environment, i.e. the lipid bilayer. Thus, the use of AFM will not only mature in basic research fields, but will also find new applications in the life science industry. 

---