Mannose paper chromatography

The use of quantitative, paper chromatography is discussed in this Section, since one of the most important factors in its application to wood-cellulose analysis is that it permits a satisfactory determination of mannan. Reasonably reliable methods for the determination of xylan have been available for some time, but mannan determinations, for the reasons discussed above, have been less satisfactory. The fact that (on chromatograms) xylose, as well as other carbohydrates, can be determined simultaneously with mannose is an added attraction in the use of this technique. 

Glucose Is the major monomer in the 72% H.SO. soluble polysaccharide and comprises about 80 to 85% or the total monomers accounted for (Table VI). Only a trace of arablnose was detected in some peel samples but xylose was found to be In measurable quantities. Several peaks on the chromatograms were found in the various components. They were not Identified positively. Peaks with retention time similar to some anomers of mannose not masked by glucose were found. Mannose has been previously noted by paper chromatography from citrus peel ( ). 

The most promising of currently available methods for the rapid, routine determination of xylose is quantitative, partition chromatography on filter paper, which permits simultaneous determination of mannose and other sugars (see p. 292). 

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