Manifold round

Figure 1. Diagram of the apparatus used in the s3mthesis and isolation of V(CO)g. Components (A) round-bottom flask, 500 mL (B) connecting tube, (C) specially adapted Schlenk tube receiver (D) stopcock (E) Dewar flask (F) water bath (G) butyl rubber vacuum tubing connection to vacuum/argon manifold. Dimensions a =165 mm b = 130 mm c = 170 mm d = 75 mm e = 20mm od.

A n-BuLi solution in hexanes (Aldrich) (1.77 M, 56.5 mL, 100 mmol) was transfered to a 500-mL, round-bottomed flask and frozen in liquid nitrogen. The flask was then evacuated and connected to a calibrated vacuum manifold. An excess of dimethylamine (HNMe2) was then condensed into the flask. The tap was closed and the mixture was allowed to warm slowly to room temperature while the flask was agitated (CARE ). Solid Li[NMe2] precipitates as the reaction proceeds. The volatiles are removed by vacuum and the white solid is pumped dry. 

Charge a two-necked, round-bottomed flask (50 mL) with copper(l) cyanide (0.1 g, 1.1 mmol), lithium bromide (0.11 g, 1.3 mmol), and a stirring bar. Fit the flask with a gas adapter (with stopcock) and a septum. Connect the flask to a vacuum/inert atmosphere manifold, and subject it to three pump/refill cycles. Add dry, distilled THF (15 mL) to the flask by use of a dry, glass syringe. Stir this mixture, and then cool it in an ice-bath. 

Electrochemical drilling (ECD) is an important application of ECM. The ECD operations enable the production of not only round holes, as in the case of mechanical drilling, but also shaped holes with oval, rectangular, triangular, and other sections to drill several tens of holes of varying diameters and/or shapes simultaneously to produce curved holes or slots, for example, ports and passages in manifolds to produce holes that are not perpendicular to the surface, and to... 

Conventional hybridization assays on membranes are known as dot blots or fine probes, depending on the geometry of the individual spots. The nucleic acids are applied with suction, using a commercially available manifold that results in a shape that is either round (dot) or elongated (line or slot). After immobilization, the membrane is incubated with complementary nucleic acid at a constant temperature, followed by one or more washes to discriminate matched from mismatched nucleic acid. The method allows multiple... 

A two-necked round-bottomed flask (250 mL) is equipped with a reflux condenser, septum cap, and magnetic stirrer bar, and connected to a supply of dry inert gas via the manifold system. The system is then evacuated and heated with a flame or a hot air gun to remove any residual trace of water and allowed to cool under nitrogen. 

While the apparatus is still hot, a two-necked round-bottomed flask (250 ml) is equipped with a Teflon-coated magnetic stirrer bar, a rubber septum on one neck whilst the second neck is connected to the double manifold. 

Into a dry two-necked, round-bottomed flask (100 mL) equipped with a magnetic stirrer bar, a septum cap, and a nitrogen gas inlet attached to the double manifold, add lithium chloride (0.02 g, 0.5 mmol) under a flow of nitrogen. Freshly distilled THF (50 mL) is added directly via a gas-tight syringe through the side-arm of the flask. 

Potassium (0.5 g, 13 mmol) is placed in a two-necked round-bottomed flask (50 ml) fitted with a rubber septum and connected to the dual manifold by a gas-inlet tube. A potassium mirror is created by evacuating the flask and heating the potassium (caution ). The flask is then placed under an atmosphere of argon and dry THF (10 mL, see Table 2.2) is added by syringe and the mixture is cooled in a dry ice/acetone mixture ( 78°C). 1,1-Diphenylethene (0.6 mL,... 

Potassium hydroxide (0.20 g 3.57 mmol) is placed in a single-necked round-bottomed flask (250 mL) equipped with a gas-inlet adapter. The flask is attached to the manifold, evacuated (to 0.01 mmHg), and heated to 70°C for 19 h. 

The monomer is prepared for addition by cooling to -1S°C in the presence of a portion of MAO. A two-necked round-bottomed flask (100 mL) fitted with a rubber septum is attached to the double manifold by means of a gas-inlet tube and flushed with argon. 1-Hexene (2 mL, 16 mmol) and MAO (0,7 mL, 12 mmol with respect to aluminium) are added using gas-tight syringes of appropriate sizes. The flask is then place in a dry ice/acetone bath and allowed to stand at 78°Cfor30 min. 

A dry two-necked round-bottomed flask (100 ml) is equipped with a Teflon -coated magnetic follower, a condenser, a gas-inlet adapter, and a septum cap. The flask is then charged with norbornene (1 g, 11 mmol). The flask is connected to the dual manifold by means of the gas-inlet adapter, and is evacuated and then maintained under an atmosphere of argon,... 

The round-bottomed flask containing the mixture is fitted with an outlet tube attached to a collecting flask (a Buchner flask with a ground-glass joint), and thence to the dual-manifold system. The system is then evacuated and flushed with argon several times. 

This mixture is then placed in a three-necked round-bottomed flask equipped with a thermometer (0-300°C), an overhead stirrer, and an outlet tube connected by (thick-walled) tubing to a Buchner flask fitted with a Quickfit (female) joint. The conical flask is, in turn, attached to the dual-manifold system using a similar outlet tube. 

To a single-necked round-bottomed flask equipped with a condenser, a Teflon-coated magnetic follower and inlet to the double manifold is added a mixture of 4,4 -difluorobenzophenone (15.0 g, 68 mmol), sodium hydroxide (5.44 g, 136 mmol), water (35 ml), and DMSO (150 ml). 

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