Manganese stabilizing protein

The flow of electrons from photosystem II to photosystem I drives the transport of protons into the thylakoid lumen. Electron transfer through the iron-sulfur proteins FeA and FeB is not understood. ° values are approximate. MSP contains a manganese cluster. (MSP = manganese stabilizing protein)... 

As 2 electrons move from each water molecule to NADP1 (blue arrows), about two H+ are pumped from the stroma into the thylakoid lumen. Two additional H+ are generated within the lumen by the oxygen-evolving complex. The flow of protons through the proton pore in CF0 drives the synthesis of ATP in CF (MSP = manganese stabilizing protein ph = pheophytin Fd = ferredoxin FNR = ferredoxin-NADP oxidoreductase)... 

Other subunits in PII include the 56- and 50-kDa antenna proteins CP43 (PsbC gene) and CP47 (PsbB gene). Three extrinsic proteins, which bind to the lumenal side of fhe thylakoid membrane, are fhe 33-kDa manganese stabilizing protein (PsbO... 

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Qian M, Al-Khaldi SF, Putnam-Evans C et al. Photoassembly of the photosystem II (Mn) cluster in site-directed mutants impaired in the binding of the manganese-stabilizing protein. Biochemistry 1997 36 15244-15252. 

Betts S, Hachigian TM, Pichersky RE et al. Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein. Plant Mol Biol 1994 26 117-130. 

Lydakis-Simantiris N, Hutchison RS, Betts SD et al. Manganese stabilizing protein of photosystem II is a thermostable, natively unfolded polypeptide. Biochemistry 1999 38 404-414. 

Motoki A, Usui M> Shimazu T et al. A Domain of the Manganese-stabilizing Protein from Synechococcus elongatus Involved in Functional Binding to Photosystem II. ] Biol Chem 2002 ... 

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Hutchison RS, Betts SD> Yocum CF et al. Conformational changes in the extrinsic manganese stabilizing protein can occur upon binding to the photosystem II reaction center an isotope editing and FTIR study. Biochemistry 1998 37 5643-5653. 

Tucker DL, Hirsh K, Li H et al. The manganese stabilizing protein (MSP) and the control of O2 evolution in the unicellular, diazotrophic cyanobacterium, Cyanothece sp. ATCC 51142. Biochim Biophys Acta 2001 1504 409-422. 

Pazos F, Heredia P, Valencia A et al. Threadii structural model of the manganese-stabilizing protein 33 kDa protein reveals presence of two possible P-sandwich domains. Proteins Struct Funct Genet 2001 45 372-381. 

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Betts SD, Ross JR, Picherslgr E et al. Mutation val35ala weakens binding of the 33-kDa manganese stabilizing protein of photosystem II to one of two sites. Biochemistry 1997 36 4047-4053. 

Popelkova H, Im M, Yocum CF. N-terminal truncations of manganese stabilizing protein identify two amino acid sequences required for binding of the eukaryotic protein to photosystem II, and reveal the absence of one binding-related sequence in cyanobacteria. Biochemistry 2002 ... 

In our laboratory we are interested in determining the structural organization of proteins associated with the Photosystem II (PS II) complex. At least seven proteins are required for oxygen evolution by PS II the 49 kDa protein CPa-1 (CP-47), the 45 kDa protein CPa-2 (CP-45), the 34 kDa protein Dl, the 33 kDa manganese-stabilizing protein, the 32 kDa D2 protein, and the 9 and 4.5 kDa subunits of cytochrome 559 proteins along... 

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