Mandelonitrile lyase

Kuroki, G.W. and Conn, E.E. (1989) Mandelonitrile lyase fromXimenia americana L. stereospecificity and lack of flavin prosthetic group. Proceedings of the National Academy of Sciences of the United States of America, 86, 6978-6981. 

Also in the (R)-class of Hnls, although of more limited application, is the (R)-mandelonitrile lyase from Phlebodium aureum [35] which catalyses the addition to some aromatic and heterocyclic carbonyls but only poorly to aliphatic carbonyls. 

MANDELONITRILE LYASE MANDELONITRILE LYASE Manganese(ll) carbonate (MnCOj), SOLUBILITY RRODUCT Manganese(ll) hydroxide (Mn(OH)2), SOLUBILITY RRODUCT Manganese ion,... 

Cheng, l.R. and Roulton, J.E. (1993) Cloning of cDNA of Prunus serotina (R)-(-l-)mandelonitrile lyase and identification of a putative FAD-binding site. Plant Cell Physiol, 34,1139-43. 

Hu, Z. and Poulton, J.E. (1997) Sequencing, genomic organization and preliminary promoter analysis of black cherry (R)-(+)-mandelonitrile lyase gene. Plant Physiol, 115(4), 1359-69. 

Wajant, H., Roerster, S., Selmar, D., Effenberger, R. and Pfizenmaier, K. (1995) Purification and characterization of a novel (R)-mandelonitrile lyase from the fern Phlebodium aureum. Plant Physiol, 109,1231-8. 

E. Wehtje, E Adlercreutz, and B. Mathiasson, Formation of C—C bonds by mandelonitrile lyase in organic solvents. Biotech. Bioeng., 36 39 (1990). 

Generally, low intensity ultrasound can increase the activity of enzymes and immobilized enzymes, or can improve the metabolism of cells by the improvement of mass transfer to substrates. One example is the enzymatic decomposition of mandelonitrile by mandelonitrile lyase in di-isopropyl ether which has been accelerated 10-fold by ultrasound [66], The mandelonitrile lyase was not denatured by 30 kHz operating at 0.5 W cm-2 and pulsed at 15 s per min. 

Alcohol oxidase P-Glucosidase and mandelonitrile lyase Rhamnosidase... 

EC4. Lyases enzymes cleaving C-C, C-O, C-N, and other bonds by other means than by hydrolysis or oxidation. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. Example mandelonitrile lyase (EC 4.1.2.10)... 

Gross, M., G. H. Jacobs, and J. E. Poulton, A rapid and sensitive spectrophotometric assay for prunasin hydrolase activity employing purified mandelonitrile lyase, Anal. Biochem., 119, 25-30 (1982). 

Lyases Addition to double bonds C=C, C=N, C=0, etc. (C=0) aldolases mandelonitrile lyase (C=C) aspartase fumarase... 

Through the years, several names and abbreviations for HNLs have been used, e.g., emulsin (for extracts from defatted almond meal), oxynitiilase, mandelonitrile lyase, MDL, MNL, and acetone cyanohydrin lyase, all with different prefixes like D, l, (R), (5), (+), (—), or a. Throughout this chapter, the designation hydroxynitrile lyase, abbreviated as HNL and the prefixes R and S, will be used. 

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