Mammalian cell surfaces, analysis

Although many proteins contain the modB carbohydrate, most of the attention has been on the analysis of two of them. One is a cell surface glycoprotein, called PsA (a.k.a. SP29), which has been cloned and sequenced. The carbohydrate modifications are located on a repeated motif, PTVT as shown by Edman degradation [68]. This sequence is typical of many of those in mammalian cell proteins which also contain O-Iinked oligosaccharides [69]. Several allelic variants of this protein from related strains of Dictyostelium discoideum contain 3-5 of these glycosylated repeats [70]. This protein... 

In this chapter, we proposed several in vitro assays using mammalian cell cultures to measure some features of metallic products. Both the colony formation assay and the viable cell number assay can detect changes in the surface conditions of metallic materials, which are difficult to detect using traditional material analyzing techniques such as microscope analysis and X-ray fluorescence spectrometric analysis. The MTT assay is more effective method to quickly analyze multiple samples at one time. These in vitro assays are applied in assessment of metallic products and understanding the biological functions of metal(s). This information is very important in order to generate environmental friendly metallic products and biocompatible ones. 

The XPS analysis of fibroblasts, epithelial cells, and smooth muscle cells washed in the absence or in the presence of serum proteins showed that they adsorb hardly any serum proteins in contrast with many materials mentioned in this section and in the section Data Interpretation and Evaluation To our knowledge, no recent studies of the surface of mammalian cells by XPS were reported. 

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