Luciferase reporter assay

Cells are lysed for Firefly and Renilla luciferase assays using the Dual-Luciferase Reporter Assay system (Promega), following the manufacturer s instructions. We use a multimode microplate reader with automatic injectors (FLUOROstar Optima from BMG Labtech, OfFenburg, Germany) for luminescence measurements. 

Table 1 Primary hit rate of different luciferase reporter assays (los loss of signal gos gain of signal) at the given hit criterion...

Westerink WM, Stevenson JC, Horbach GJ, Schoonen WG (2010) The development of RAD51C, Cystatin A, p53 and Nrf2 luciferase-reporter assays in metabolically competent HepG2 cells for the assessment of mechanism-based genotoxicity and of oxidative stress in the early research phase of drug development. Mutat Res 696 21 0... 

The luciferase reporter assay captures the effect of test compounds on downstream signaling events (i.e., activation of NFAT and AP-1). However, such effects could also be due to off-target activity and not (solely) inhibition of the PTP of interest. If the direct substrate(s) of the target PTP is known, and phospho-specific antibodies for this/these substrate(s) are available, immunoblot assays can be employed that directly probe the phosphorylation levels of the substrate(s). If no direct substrate of the target PTP has been... 

Sherf BA, Navarro SL, Hannah RR (1996) Dual-luciferase reporter assay an advanced co-reporter technology integrating firefly and renilla luciferase assays. Promega Notes 57 02... 

Upon lipopolysaccharide stimulation (1 tg/ml), Prabhu et al. (2002) found significantly higher iNOS transcript and protein expression levels with an increase in NO production in selenium-deficient RAW 264.7 cells than in the Se-complemented cells. Electrophoretic mobility-shift assays, nuclear factor-xB-luciferase reporter assays and Western blot analyses indicated that the increased expression of iNOS in Se deficiency could be due to an increased activation and consequent nuclear localization of the redox-sensitive transcription factor NF-xB. 

Fig. 6.3 Comparison of the in vitro proliferator-activated receptor (PPARy) transactivation activity, measured with a luciferase reporter assay, between a full agonist (represented by squares) and a partial agonist (represented by triangles)...

Lum LD, Kulak O (2012) Multiplexed luciferase reporter assay systems. US Patent 12/ 908,754... 

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