LSCM images reconstruction

D visualization of bicontinuous morphologies in block copolymer systems has been achieved [26-27] by TEMT (see Sect 2.2). This technique affords the real-space structural analysis of complex nanoscale morphologies without a priori synunetry or surface assumptions [97]. Application of numerical methods developed [39, 98] to measure interfacial curvatures from 3D LSCM images of SD polymer blends (see Sects. 3.2.3 and 4.3.3) to a TEMT reconstruction of the G morphology yields the first experimental measurements of interfacial curvature distributions, as well as (H) and an, in a complex block copolymer nanostructure. 

Figure 20.1 Schematic of LSCM. A stack of 2D images is recorded at different focal planes in the specimen and subsequently reconstructed in three dimensions using the marching cubes algorithm [136], See insert for the color representation of the figure.)...

SBR/PB phase-separating structure, reconstructed from 40 slices of image-processed LSCM scans (b) comparison between structure factors S q/qm) obtained... 

The 2D LSCM slices were binarized with the aid of computer image analysis and were stuck together to make a 3D representation of the structures (see Sect. 3.1). The MCA was used to model the interface between the DPB and PB components by contiguous polygons in the reconstruction process. The interfacial area was estimated by summing the area of the polygons, from which the interfacial area per unit volume, S(f)> was estimated. The volume fraction of one of the phases, 0, was also measured. The volume... 

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