Ion-exchange chromatography, low pressure

This approach has been used extensively for amino acid analysis using low-pressure ion-exchange chromatography and post-column ninhydrin reaction. Spraying, dipping and vapour-treatment techniques are well known as post-separation reactions in TLC, but these are considered only briefly since the majority of them are not quantitative. While the problems of pre-separation techniques are quite similar for TLC and HPLC, they differ considerably for post-separation reactions. 

Levison PR, Mumford C, Sweater M, et al. (1997) Performance comparison of low-pressure ion-exchange chromatography media for protein separation. Journal of Chromatography A 760 151-158. 

Ion-Exchange Chromatography. Low pressure GPC has almost universally replaced low pressure ion-exchange chromatography. However, the basic principles, which in most cases do not reflect true ion-exchange, have been refined and incorporated into HPLC. 

This chapter summarizes the majority of the literature on ion exchange chromatography in biochemistry over the five years preceding 1981. Occasionally some important older works are cited (especially reviews or monographs). It can be seen from the literature that even today many authors are satisfied with the use of older, classically proven, but time consuming, low pressure separation methods and develop them further. On the contrary, at the same time a broad shift to modern trends can be observed, which is represented by numerous applications of the rapid medium and high pressure liquid chromatography in various fields of biochemistry. Therefore both approaches must be discussed here. 

Most separations in liquid chromatography are performed at room temperature for convenience and because ambient temperatures provide reasonable column efficiency for low molecular mass solutes. Elevated temperatures are commonly used in ion-exchange chromatography to improve mass transfer kinetics and in size-exclusion chromatography to provide adequate solubility for polymers in useful mobile phases. Wider interest in temperature control and high-temperature separations in general results from improved precision of retention measurements (section 1.1.1), greater column efficiency (section 1.5.2), the use of temperature as a variable for method development (section 4.4.4), and shorter separation times due to the more favorable use of the column inlet pressure [70,71]. 

Ion Exchange Resins - Spectra/Gel Ion Exchange resins are ion exchange media for use in low-pressure liquid chromatography. They are based on a polystyrene/divinylbenzene support and are available for both anion and cation exchange applications. This site will give you a reasonable... 

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