Liver basolateral membrane

Zimmerli, B., J. Vaiantinas, and P. J. Meier. Multispecificity of Na+-dependent taurocholate uptake in basolateral (sinusoidal) rat liver plasma membrane vesicles. J. Pharmacol. Exp. Ther. 1989, 250, 301-318. 

OATP-C, a liver-specific member of this transporter family, was cloned by number of groups [17, 30-32] and often referred by aliases such as liver-specific transporter 1 (LST-1) and OATP2. Immunohistochemical analysis proved OATP-C expression on the basolateral membrane of hepatocytes [32]. As with OATP-A, OATP-C... 

Polarized tissues directly involved in drug absorption (intestine) or excretion (liver and kidney) and restricted drug disposition (blood-tissue barriers) asymmetrically express a variety of different drug transporters in the apical or basolateral membrane resulting in vectorial dmg transport. This vectorial dmg transport is characterized by two transport processes the uptake into the cell and subsequently the directed elimination out of the cell (Figure 15.3). Because the uptake of substances... 

Octl (Slc22al) is expressed in the liver and kidney (112,113), while OCT1 is expressed predominately in the kidney (114). Octl is localized to the sinusoidal membrane of the hepatocytes surrounding the central vein and basolateral membrane in the kidney (115,116). Although the membrane localization has not been determined, Octl is likely expressed in the basolateral membrane of the small intestine since the distribution of metformin and intestinal excretion of tetrae-thylammonium (TEA) following intravenous injection was decreased in Octl(-/-) mice (117,118). The reduction in the distribution of metformin in Octl(-/-) was the most prominent in the duodenum followed by jejunum and ileum, but unchanged in the colon, which was consistent with the mRNA of Octl distribution from... 

MRP4 is abundantly expressed in the kidney followed by the liver (238,260). The membrane localization of Mrp4 is tissue dependent sinusoidal membrane in the hepatocytes (261), brush border membrane of the renal tubules (262,263), luminal membrane of the brain capillaries (262), and basolateral membrane of the choroid epithelial cells (262). 

Meier PJ, St. Meier-Abt A, Barrett C, et al. Mechanisms of taurocholate transport in canalicular and basolateral rat liver plasma membrane vesicles. Evidence for an electrogenic canalicular organic anion carrier. J Biol Chem 1984 259 10614—10622. 

Shoji T, Suzuki H, Kusuhara H, et al. ATP-dependent transport of organic anions into isolated basolateral membrane vesicles from rat intestine. Am J Physiol Gastrointest Liver Physiol 2004 287 G749-G756. 

Although we have assumed that there is no mucosal diffusional barrier for the CYP3A substrate, the unbound inhibitor concentration in the intestinal epithelia (7gm) may or may not be equivalent to that in plasma and the liver (7U). 7gm may exceed the unbound portal plasma concentration during the inhibitor absorption phase or be less than the unbound portal concentration postabsorption if there is not rapid equilibrium between the intracellular and portal plasma compartments (i.e., a basolateral membrane diffusional barrier exists). This obviously makes it challenging to anticipate the quantitative effect of an inhibitor on intestinal first-pass metabolism. 

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