Liquid chromatography/mass spectrometry LCMS

Strategies for the Removal of Ionic and Non-Ionic Detergents From Protein and Peptide Mixtures For On- And Off-Line Liquid Chromatography Mass Spectrometry (LCMS)... 

Liquid chromatography mass spectrometry (LCMS) may be performed with a PE Sciex API 300 electrospray triple-quadrupole mass spectrometer, and an Applied Biosystems model 130 HPLC system with a 5 x Vydac C18 column (1 x 250 mm, using aqueous tnfluoroacetic acid/acetonitrile solvents), or equivalent... 

Synthesis of heterocycle 31" The resin, in a well of a 96-well reaction block, was treated with 100% formic acid (1.2 mL) for 18 hours at room temperature. After this time, the resin was filtered and the filtrate was concentrated under vacuum to yield heterocycle 31 as a film. The product was diluted with H20 MeCN (50 50), freeze-dried, and purity determined by liquid chromatography-mass spectrometry (LCMS) without further purification. 

LCMS Liquid chromatography mass spectrometry, sometimes LC-MS... 

Whitelegge, J.P, Zhang, H., Aguilera, R., Taylor, R.M., Cramer, W.A. (2002). Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosus. Mol. Cell. Proteomics. 1, 816-827. 

A single capillary scale reverse phase high-performance liquid chromatography system coupled online to automated electrospray ion trap tandem mass spectrometry (ID-LCMS) is usually performed to characterize the protein fractions. The following protocol is an example of the implementation of the method to identify the peptide components of tryptic digests of the yeast HPLC fractions analysis using the ion trap tandem mass spectrometer instrument described in Section 13.2.2.5, and typical results are shown in Figure 13.2-3. 

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