Liposomes, in gene therapy

Lasic DD, Templeton NS (1996) Liposome in gene therapy. Adv Drug DeUv Rev 20 221-266... 

Lasic DD, Vainer JJ, Working PK. Sterically stabilized liposomes in cancer therapy and gene delivery. Curr Opin Mol Ther 1999 1 177-185. 

Table 11.2. Vector systems used to deliver genes into mammalian cells. To date, the majority of clinical trials undertaken have utilized retroviral vector systems. Non-viral systems have generally been employed least often, although some, e.g. nucleic acid-containing liposomes, may be used more extensively in the future. Some of the methods tested, e.g. calcium phosphate precipitation, electroporation and particle acceleration, are unlikely to be employed to any great extent in gene therapy protocols...

Zhu, J., Zhang, L., Hanisch, U. K., Feigner, P. L., and Reszka, R. (1996b). In vivo gene therapy of experimental brain tumors by continuous administration of DNA-liposome complexes. Gene Therapy, 3,472-6. 

Farhood, H., Serbina, N. and Huang, L. (1995) The Role of dioleoylphospatidylethanolamine in cationic liposome mediated gene therapy. Biochim. Biophys. Acta., 1235, 289-295. 

Simoes, S., Pedro, P., Duzgunes, N. and Pedrosa de Lima, M.C. (1999) Cationic liposomes as gene transfer vectors Barriers to successful application in gene therapy. Curr. Opin. Struct. Biol, 1, 147-157. 

Duffels, A., Green, L.G., Ley, S.V. and Miller, A.D. (2000) Synthesis of high-mannose type neoglycolipids Active targeting of liposomes to macrophages in gene therapy. Chem. Eur. J., 6,1416-1430. 

Lasic, D.D. (1997a) Recent developments in medical applications of liposomes sterically stabilized liposomes in cancer therapy and gene delivery in vivo. J. Controlled Release, 48,203-222. 

V. J. Dzau, M. Mann, R. Morishita, and Y. Kaneda, Fusigenic viral liposome for gene therapy in cardiovascular diseases, Proc. Natl. Acad. Sci. USA 93 11421 (1996). 

Liposomes. These are small membrane vesicles that can be used to encase the vector they fuse with the cell membrane (and sometimes the nuclear membrane too), delivering the DNA into the cell. This approach is particularly useful for delivering genes to cells in vivo (such as in gene therapy). 

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