Lipoproteins serum analytical methods

Medicine. There are many applications of PEI in the medical sector. Analytical methods, such as the quantitative determination of the surface charge of serum lipoproteins (442), are aided by use of PEI, and it is also used as a carrier in the development of polymer drugs (443,444). 

ANALYTICAL METHODS OF SERUM LIPOPROTEINS BY HPLC 2.1. Column... 

Analytical methods employing paper electrophoresis (for bibliography see Pezold 1961) have been used in lipoprotein research since the introduction of Sudan Black B as a Upid stain (Swahn 1952). Paper electrophoresis is a deceptively simple technique. The analysis of electrophoretic data is more complex. While a number of important studies have been published, it has sometimes been difficult to interpret data showing arbitrary ratios between partially separated and poorly characterized serum lipoproteins whose concentrations were estimated by non-specific staining. 

Advantage is taken of the variation in density in the design of ultracentrifugal analytical methods for the determination of serum lipoproteins. These lipoproteins range in hydrated density from less than 1.0 g./ml. up to 1.145 g./ml. The quantitative determination of these lipoproteins is greatly facilitated if they are first separated from the serum proteins (hydrated densities of approximately 1.3 g./ml.). The technique of effecting the separation of the lipoproteins from the proteins depends upon which segment of the entire lipoprotein spectrum is of interest. This... 

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