Lipid pharmaceutical products analysis

Like ELSD, CAD has been applied for the analysis of nonvolatile neutral, acidic, basic, and zwitterionic compounds, both polar and nonpolar. Compound classes include amino acids, fatty acids, carbohydrates, lipids, proteins, steroids, surfactants, and other compounds with weak or no chromophores used in the pharmaceutical, food, chemical, and consumer products industries. Some applications involving ionic and polar species are described below. 

HPLC has been used for measuring various compounds, for example, carbohydrates, vitamins, additives, mycotoxins, amino acids, proteins, triglycerides in fats and oils, lipids, chiral compounds, and pigments. Several sensitive and selective detectors such as ultraviolet-visible, FL, electrochemical, and diffractometric are available to utilize with HPLC depending on the compound to be analyzed. Various HPLC methods based on these detectors have been published for the measurement of vitamin E in biological and pharmaceutical samples and food products. Excellent literature reviews of HPLC based on various detectors in the analysis of vitamin E content in various matrices have been reported (Abidi, 2000 Aust et al., 2001 Ruperez et al., 2001 Lai and Franke, 2013). Table 19.5 reports several recent HPLC methods for the analysis of vitamin E and similar compounds in various matrices. 

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