Lipid microsomal

Tablel Incorporation of [ Cloleate into microsomal lipids. Microsomes were incubated with ( C] 18 1 CoA (50 nmoles) and after incubation the distribution in complex lipids determined.

Kitada, M., Igarashi, K., Hirose, S. Kitagawa, H. (1979). Inhibition by polyamines of lipid peroxide formation in rat liver microsomes. Biochemical Biophysical Research Communications, 87, 388-92. 

The mechanisms involved in the establishment of lipid asymmetry are not well understood. The enzymes involved in the synthesis of phospholipids are located on the cytoplasmic side of microsomal membrane vesicles. Translocases (flippases) exist that transfer certain phospholipids (eg, phosphatidylcholine) from the inner to the outer leaflet. Specific proteins that preferentially bind individual phospholipids also appear to be... 

With the exception of the acetyl-CoA thiolase, all these enzymes are located exclusively in the peroxisomes, whereas the enzymes that are involved in lipid synthesis are found in the microsomes and the mitochondrion. 

Poli, G., Dianzani, M.U., Cheeseman, K.H., Slater, T.F., Lang, J. and Esterbauer, H. (1985). Separation and characterization of the aldehydic products of lipid peroxidation stimulated by carbon tetrachloride or ADP-iron in isolated rat hepatocytes and rat liver microsomal suspensions. Biochem. J. 227, 629-638,... 

Kramer, J.H., Mak, I.T. and Weglicki, W.B. (1984). Differential sensitivity of canine cardiac sarcolemmal and microsomal enzymes to inhibition by free radical-induced lipid peroxidation. Circ. Res. 55, 120-124. 

Microsomal chemiluminescence (lipid peroxidation) is stimulated by oxygenation in the presence of anaerobicaUy released ferritin iron. Fed. Proc. 45, 174. 

Wills, E.D. (1969). Lipid peroxide formation in microsomes the role of non-heme iron. Biochem. J. 113, 325-332. 

Koster, J.R. and Slec, R-G. (1980). Lipid peroxidation of rat liver microsomes. Biochem. Biophys. Acta 620, 489-499. 

Pederson, T.C., Buege, J.A. and Aust, S.D. (1973). Microsomal electron transport. The role of reduced nicotinamide dinucleotide phosphateliver microsomal lipid peroxidation. J. Biol. Chem. 248, 7134—7141. 

Castillo, T., Koop, D.R., Kamimura, S., Triadafilopoulos, G. and Tsukamoto, H. (1992). Role of cytochrome P-450 2E1 in ethanol-, carbon tetrachloride- and iron-dependent microsomal lipid peroxidation. Hepatology 16, 992-996. 

Cheeseman, K.H., Proudfoot, K., Maddix, S.P., Collins, M.M., Milia, A. and Slater, T.F. (1985). Low rate of NADPH/ADP-iron dependent lipid peroxidation in hepatic microsomes of DBA/2 mice. FEBS Lett. 184, 343-346. 

Corongiu, F.P., Lai, M. and Milia, A. (1983). Carbon tetrachloride, bromotrichloromethane and ethanol acute intoxication. New chemical evidence for lipid peroxidation in rat tissue microsomes. Biochem. J. 212, 625-631. 

Slater, T.F. (1967). Stimulatory effects of carbon tetrachloride in vim on lipid peroxidation in rat liver microsomes. Proc. 4th FEBS Meeting, Oslo, abstract no. 216, 67. 

Fontecave, M., Mansuy, D., Jaouen, M. and Pezerat, H. (1987). The stimulator) effects of asbestos on NADPH-dependent lipid peroxidation in rat liver microsomes. Biochem. J. 241, 561-565. 

Gulumian, M., Sardianos, F., Kilroe-Smith, T. and Ockerse, G. (1983). Lipid peroxidation in microsomes induced by crocidolite fibres. Chem. Biol. Interact. 44, 111-118. 

The phenothiazines, chlorpromazine and promethazine, have been described as inhibitors of CCU-induced lipid peroxidation at relatively high concentrations in rat liver microsomes (Slater, 1968). Structural modifications of chlorpromazine were undertaken to try to increase antioxidant activity and maintain molecular lipophilicity. The 2-N-N-dimethyl ethanamine methanesulphonate-substituted phenothiazine (3) was found to be a potent inhibitor of iron-dependent lipid peroxidation. It was also found to block Cu -catalysed oxidation of LDL more effectively than probucol and to protect primary cultures of rat hippocampal neurons against hydrogen peroxide-induced toxicity in vitro (Yu et al., 1992). 

Battioni, )., Fontecave, M., Jaouen, M. and Mansuy, D. (1991). Vitamin E derivatives as new potent inhibitors of microsomal lipid peroxidation. Biochem. Biophys. Res. Commun. 174, 1103-1108. 

Slater, T.F. (1968). The inhibitory effects in vitro of phenothia-zines and other drugs on lipid-peroxidation systems in rat liver microsomes, and their relationship to the liver necrosis produced by carbon tetrachloride. Biochem. J. 106, 155-160. 

Li, CM, Presley, B, Zhang, XM, Dashti, N, Chung, BH, Medeiros, NE, Guidry, C, and Curcio, CA, 2005. Retina expresses microsomal triglyceride transfer protein Implications for age-related maculopathy. J Lipid Res 46, 628-640. 

Peroxyl radicals are the species that propagate autoxidation of the unsaturated fatty acid residues of phospholipids (50). In addition, peroxyl radicals are intermediates in the metabolism of certain drugs such as phenylbutazone (51). Epoxidation of BP-7,8-dihydrodiol has been detected during lipid peroxidation induced in rat liver microsomes by ascorbate or NADPH and during the peroxidatic oxidation of phenylbutazone (52,53). These findings suggest that peroxyl radical-mediated epoxidation of BP-7,8-dihydrodiol is general and may serve as the prototype for similar epoxidations of other olefins in a variety of biochemical systems. In addition, peroxyl radical-dependent epoxidation of BP-7,8-dihydrodiol exhibits the same stereochemistry as the arachidonic acid-stimulated epoxidation by ram seminal vesicle microsomes. This not only provides additional... 

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