Linum usitatissimum, hydroxynitrile lyases

Roberge, C., Fleitz, F., Pollard, D. and Devine, P., Synthesis of optically active cyanohydrins from aromatic ketones evidence of an increased substrate range and inverted stereoselectivity for the hydroxynitrile lyase from Linum usitatissimum. Tetrahedron Asymm., 2007,18, 208. 

HNLs comprise a heterogenous enzyme family, since hydroxynitrile lyase activity has evolved in different structural frames by convergent evolution [17, 18]. Thus, (S) -specific HNLs based on an a/P-hydrolase fold framework from Manihot esculmta (cassava) [19-21], Hevea hrasilensis (rubber tree) [22-26], and Sorghum hicolor (millet) [27-33] have been described. (R)-specific HNLs based on the structural framework of oxidoreductases were isolated from Linum usitatissimum (flax) [30, 34-37] and Rosaceae (e.g., bitter almonds) [31, 38]. Despite their potential in biocatalysis only few HNLs (from cassava and rubber tree) are available by recombinant gene expression, which is a prerequisite for their technical application [20, 24]. Thus, cloning, recombinant expression, and... 

H. Breithaupt, Klonierung und Expression der (R)-Hydroxynitrile Lyase aus Linum usitatissimum (Lein) [Doctoral Thesis], Heinrich-Heine University Dtisseldorf 1997. 

Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)./. Mol. Catal. B -Enzymaticl999, 6, 315-332. 

K. Trummler, H. Wajant, Molecular cloning of acetone cyanohydrin lyase from flax Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases./. Biol. Chem. 1997, 272, 4770-4774. 

Change of product configuration owing to a priority replacement according CIP rules Abbreviations HNL, hydroxynitrile lyase P. a., Prunus amygdalus S. b., Sorghum bicolor, H. b., Hevea brasi-liensis M. e., Manihot esculenta I. u., Linum usitatissimum. 

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