Linked scanning application

MS", application of successive mass spectrometric measurements n of them), particularly in linked scanning of m/z, which is the ratio of the mass (m) of an ion and the number of charges (z) on it. Older publications used m/e, but as e is the actual charge on an electron and not the number of charges on the ion, the use of m/e was abandoned, m/z. mass-to-charge ratio, a measure of molecular mass PDB. PeeDee Belemnite (a carbon isotope standard see VPDB)... 

Millington DS, Roe CR, Maltby DA (1984) Application of high resolution fast atom bombardment and constant B/E ratio linked scanning to the identification and analysis of acylcarnitines in metabolic disease. Biomed Mass Spectrom 11 236-241... 

Another application is the differentiation of closely related isomers. The conventional mass spectra of butobarbitone and seebutobarbitone are qualitatively identical (Figs 5A and 5B). The B/E linked scans of their (M—29) ions (m/z 183) shown in Figs 5C and 5D... 

These examples show the applications of MIKE method. In the same way, linked scan techniques may be particularly valuable. These methods are rapid, thus allowing the accumulation of iterative results, since there is a satisfying reproducibility. 

G. A. Warburton, A. G. Brenton, J. R. Chapman, and K. T. Taylor, Application of B/E linked scanning to structural differentiation using MS80-DS55. Kratos Data Sheet, No. 134 (1981). 

Direct MS analysis of oligomer mixtures by FAB can be combined with the Collision Induced Dissociation (CID) technique, which is used to induce the fragmentation of selected molecular ions by means of collision with an inert gas (MS/MS). This allows one to obtain daughter ions classical MS/ MS techniques utilize the linked scanning of two or more mass analyzers to obtain product (daughter) and/or precursor (parent) ion spectra see Chapters 1 and 4. Systematic application of tandem Mass Spectrometry (MS/ MS) has produced important results in the analysis of polymeric materials by FAB. " ... 

We are confident that any user of this combined evaluation technique, as well as the development of future test standards for manual ultrasonic testing will benefit from this result, because it allows a greater flexibility in the applicable method without loosing reliability. Often an expensive production of a reference block can be avoided and therefore testing costs are reduced. Since all calculations are performed by a PC, the operator can fully concentrate on his most important duty scanning the workpiece and observing the A-scan. Additional time will be saved for the test documentation, since all testing results are stored in the instrument s memory (the PC s hard drive) with full link to the Software World (Microsoft Word, Excel, etc.). 

A special application of the peptide scan is the hybritope scan (hybrid-epitope) (44,45). In order to increase the affinity by extention of the peptide sequence in a hybritope scan, several mixtures of amino acids at positions flanking the related sequence of the protein are introduced. Another strategy to optimize a peptide scan is the so-called duotope scan (duo-epitope) (46). For a duotope scan one generates the peptide scan twice and combines the sequence of both arrays linked via a spacer, such as two -alanine molecules. In this way it is possible to avoid possible steric hindrance and to increase activity by extention of the active sequence. The pattern of such a duotope scan resembles that of a combinatorial library with the exception that each field represents a combination of two sequences rather than two amino acids. 

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