Linkage region fragment

Application to a Linkage Region Fragment from Connective Tissue Proteogfycans... 

MOLECULAR STRUCTURE OF THE CARBOHYDRATE-PROTEIN LINKAGE REGION FRAGMENTS FROM CONNECTIVE-TISSUE PROTEOGLYCANS... 

R421 N. R. Krishna and P. K. Agrawal, Molecular Structure of the Carbohydrate-Protein Linkage Region Fragments from Connective-Tissue Proteoglycans , Adv. Carbohydr. Chem. Biochem., 2001, 56, 201... 

Rama Krishna N, Agrawal PK. Molecular structure of the carbohydrate-protein linkage region fragments from connective tissue proteoglycans. Adv Carbohydr Chem Biochem 2000 56 201-234. 

Fig. 5. —Tentative Model for a Heparin Chain Containing the Linkage Region. [1 and 2 are sites of cleavage with 2-pyridone, affording fragments A and B A may be further cleaved by a more drastic treatment. The active site for antithrombin is proposed - 8 to be within fragment A. ...

These conformations were subjected to a combined MD/EM refinement using the NOESY derived constraints as pseudoenergy functions. Families of conformations for the whole molecule were then constructed from the structures derived for each linkage. This procedure was demonstrated on a fragment from the carbohydrate-protein linkage region of connective tissue proteoglycans. 

We will illustrate our methodology using 0-3-D-galactopyranosyl -(1->4) 0-3 D-xylopyranosyl-(1->0)-L-serine (GXS), a fragment from the carbohydrate-protein linkage region of xylose/serine linked proteoglycans. 

Oligosaccharide Fragments Isolated from the Carbohydrate-Protein Linkage-Region of Dermatan Sulfate Proteoglycans 1... 

The constitution of pleiomutine (230), apart from the decision in favour of C(15) or C(17) as a linkage position, can be established from the mass spectrum of the alkaloid. In the low-mass region fragments mje 278,249, and 208, characteristic of eburnamenine,are apparent, as well as the previously mentioned fragments V and w and the — 28 peak, all typical of pleiocarpinine (232). 

The proof of this sequence, and of the nature of the carbohydrate-peptide bond, was based on enzymic erosion to a glycopeptide fragment that was then studied by adaptations of classical methods. A similar linkage-region probably occurs in heparin,86 heparitin sulfate,87 dermatan sulfate88 and chondroitin 6-sulfate.88a It is possible that the polypeptide core itself is branched.89... 

Several fragments of the carbohydrate-protein linkage region... 

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