Lineweaver, Hans

In 1934, Dean Burk (1904-88) and Hans Lineweaver (1907- ), at the United States Department of Agriculture, proposed an extremely simple mathematical solution to this experimental problem. By simply plotting the reciprocals of... 

For the convenience of graphic presentation of experimental data Hans Lineweaver (1907-2009) and Dean Burk (1904-1988) modified equation... 

Hans Lineweaver and Dean Burk, emphasized the uncertainty of large l/v values and suggested to apply smaller weightings for these values. Problems arise specifically for small substrate concentrations resulting in slow reaction rates. When the reaction rates are particularly small 1) the reciprocal value becomes very large, as does the error of this value. 

Characteristic Quantities In principle, the quantities and ro,max that characterize an enzyme can be determined by directly fitting the Michaelis-Menten equation to the measured data using computer supported methods of nonlinear regression. We can simplify the analysis in a manner suggested by Hans Lineweaver and Dean Burke in 1934 by linearizing the relation. In order to do this, we must find the reciprocal of the Michaelis-Menten equation. After transforming, we have ... 

Lineweaver-Burk plot A double-reciprocal plot used to determine the two constants featured in simple enzyme kinetic equations such as Michaelis-Menten kinetics, Monod kinetics, and in similar adsorption isotherm models such as the Langmuir adsorption isotherm. The constants are determined from the intercept with the y-axis and the gradient (see Fig. 26). It was devised and published in 1934 by American chemist Hans Lineweaver (1907-2009) and American biochemist Dean Burk (1904-1988). 

Prior to the development of rapid methods for data treatment, Hans Lineweaver and Dean Burk proposed a simple method using the reciprocals of eq. (14.13) to obtain experimentally the values of and Ky [4],... 

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