Limit, Peak Capacity, Resolution Possibilities for Gradient Optimization

Detection Limit, Peak Capacity, Resolution Possibilities for Gradient Optimization 

The three main objectives of optimization are low detection limit, good separation and, not in the least, fast separation. We start with the detection limit. Perhaps, this task is the easiest - at least as far as the implementation of 

Use a short, steep gradient with higher final concentration of %B. The steeper the gradient, the sooner the eluent brings the components to the column outlet and the lower the volume of mobile phase needed will be. 

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