Light source spectrum

Spectra. The spectrum of the arc light sources were measured using a LiCor LI-1800 speetroradiometer, and are shown on Figure 2. No appreciable change in the light source spectrum was observed in the first 18 months of operation. 

Heat protection filter CALFLEX / Light source spectrum Xenon lamp. 

Two scans are required to obtain an absorption spectrum. First, a blank reference scan is taken that characterizes the broadband light source. Then a scan with the sample in place is recorded. The ratio of the sample power spectrum to the reference power spectrum is the transmission spectrum. If the source has stable output, then a single reference scan can be used with many sample scans. 

A8, which leads to D, = 1/(2A8). The factor of two arises because a minimum of two data points per period are needed to sample a sinusoidal wavefonn. Naturally, the broadband light source will detennine the actual content of the spectrum, but it is important that the step size be small enough to acconunodate the highest frequency components of the source, otherwise they... 

Figure 15-12. Spectrally resolved pliotocurrent of a ITO/PPV/Mg photodiode at dilTcrcnt bias after correction for dark current, light source, and monochromator response, and normalization to the same peak value. The broken line is the normalized absorption spectrum of PPV (reproduced by permission of the Institute of Physics from Ref. 143)).

Clearly, unless monomer is the intended photoinitiator, it is important to choose an initiator that absorbs in a region of the UV-visible spectrum clear from the absorptions of monomer and other components of the polymerization medium. Ideally, one should choose a monochromatic light source that, is specific for the chromophorc of the photoinitiator or photosensitizer. It is also important in many experiments that the total amount of light absorbed by the sample is small. Otherwise the rate of initiation will vary with the depth of light penetration into the sample. 

The main problem in developing fibers for IR transmission is that silica glass is not transparent in that area of the spectrum. Suitable materials include the selenides and other chalcogenide glasses (particularly for the CO2 laser light source) and the fluorohafnate glasses. In addition, materials, such as zirconium fluo-... 

Current instruments use complex and refined optical systems in order to radiate the cuvette with monochromatic light selected from the radiant spectrum of the light source. A radically different approach may be practical when tunable lasers become available at reasonable prices. 

Our short review of standardization procedures starts again with illumination. Figure 1.4 shows the dispersed spectrum of the light source which is most relevant for observers daylight. Most instruments use incandescent lamps that emit very weakly in the blue and UV regions and are therefore well suited in situations where no fluorescence should be excited. 

The simplest fluorescence measurement is that of intensity of emission, and most on-line detectors are restricted to this capability. Fluorescence, however, has been used to measure a number of molecular properties. Shifts in the fluorescence spectrum may indicate changes in the hydrophobicity of the fluorophore environment. The lifetime of a fluorescent state is often related to the mobility of the fluorophore. If a polarized light source is used, the emitted light may retain some degree of polarization. If the molecular rotation is far faster than the lifetime of the excited state, all polarization will be lost. If rotation is slow, however, some polarization may be retained. The polarization can be related to the rate of macromolecular tumbling, which, in turn, is related to the molecular size. Time-resolved and polarized fluorescence detectors require special excitation systems and highly sensitive detection systems and have not been commonly adapted for on-line use. 

Generally the first thing to be done in preparation for the photochemical study of a compound is to determine the visible and ultraviolet absorption spectrum of the compound. Besides furnishing information concerning the nature of the excited state potentially involved in the photochemistry (see Section 1.4), the absorption spectrum furnishes information of a more applied nature as to the wavelength range in which the material absorbs and its molar absorptivity e. From this information it is possible to decide what type of light source to use for the irradiation, what solvents can be used to... 

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