Ligands and Receptors Involved in Platelet Aggregation

3 Flow cytometry for measuring receptors/Ugands number and quality 

1 Parallel studies of platelet surface receptors/ligands and platelet aggregation 

Capture efficiency, a, is defined as the fiaction of shear-induced collisiorrs between two platelets that result in doublets, i.e., the ratio of the rate of the measured initial aggregate formation for dilute suspensions (dN/dt) to the frequency of two-body coUisiorts in the suspension. 

The rate of platelet aggregation for spherical particles of equal size in suspension and colliding only via Brownian motion is related to the capture efficiency, o (B), as 

The Smoluchowski collision frequency of a suspension, CF, is given by equation 2 CF = 16/3 (G N/ a ), where G is the shear rate and a is the equivalent spherical radius of platelet. Therefore the capture efficiency can be expressed as Eq. 3  

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