Ligand identification

Klekota B, Miller BL, Dynamic diversity and small-molecule evolution a new paradigm for ligand identification, Trends Biotechnol., 17 205-209, 1999. 

Figure 7-12. Diagram of the automated ligand identification system (ALIS) affinity selection-mass spectrometry method. (Reprinted from reference 85, with permission of Elsevier Science.)...

Strategies for Ligand identification for Orphan GPCR Receptors... 

The new paradigm for orphan receptor-based drug discovery is shown diagrammatically (Fig. 5). A first step toward ligand identification for a given orphan... 

Functional Assays Used for Orphan Receptor Ligand Identification... 

Because of these powerful qualitative capabilities, FTICR mass spectrometry is especially useful for characterization of DCLs. Because the composition of the DCL cannot be statically defined, except when in equilibrium with the receptor, it is essential to be able to identify the components at this stage. Using FTICR MS, identification can be done by a combination of exact mass determination and tandem-in-time mass spectrometry to yield par-ent/daughter ion structural information. Although FTICR is an expensive option for combinatorial library characterization, it can provide the most direct information for ligand identification in libraries. 

K.H. Bleicher, L.G. Green, R.E. Martin, M. Rogers-Evans, Ligand identification for G-protein-coupled receptors a lead generation perspective, Curr. Opin. Chem. Biol. 

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