Library-based sequencing

Reineke, U., Kramer, A., and Schneider-Mergener, J. (1999). Antigen sequence- and library-based mapping of linear and discontinuous protein-protein interaction sites by spot synthesis. Curr. Top. Microbiol. Immunol. 243, 23-36. 

Yang, J.P. et al. 2006. A novel RNAi library based on partially randomized consensus sequences of nuclear receptors identifying the receptors involved in amyloid-/ degradation. Genomics 88, 282-292. 

Over the past few years, a number of strategies have been developed to screen random peptide libraries for sequences targeting specific proteins. Peptides with desired binding properties can be serially selected and amplified based on a physical link with the encoding cDNA. These technologies include mRNA/DNA, viral, ribosomal, and cell displays with phage display being by far the most commonly used and probably the best understood. 

Platform Library Generation/ Sequencing Principle Read Length (Bases) Runtime (Days)/Gb per Run Pros Cons Biological Applications... 

Assembly and transformation of polysaccharides by molecular-scale tools is triggered by principles of self-organizing complex systems and include enzymatically catalyzed nonlinear formation/transformation and degradation based on molecular-level library command sequences (genetic code). 

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