Read length

Beyond the speed and cost considerations of each sequencing technology, parameters including read length, sampling depth, and primer selection must... 

Advantage - Cost effectiveness - Massive throughput - Low hands-on time - Long-read length improves mapping in repetitive regions - Short-run time - Low error rate - Massive throughput - Short-run times - No need for modified DNA bases... 

When a longer CE channel (40 cm) on a glass chip was used, the average DNA read length (four-color-detection) was successfully increased to 800 bases in 80 min (98% accuracy) for M13mpl8 DNA (or the DNA with a 2-kbp human chromosome 17 insert). For comparison, a commercial capillary-based DNA sequencer took 10 h to complete the same 800-base analysis [968]. Moreover, DNA sequencing up to 700 bp (with 98% accuracy) has been achieved in 40 min using an 18-cm plastic microchannel [969]. 

In a later report, a 96-lane CE glass chip was used to carry out DNA sequencing. Tapered turns were employed to increase the channel length, and hence the DNA read length [972], More recently, a 384-lane plate for DNA sequencing has also been reported [973],... 

Currently this technique produces short reading lengths, which make it indicated for resequencing applications in which a reference sequence is... 

Platform Library Generation/ Sequencing Principle Read Length (Bases) Runtime (Days)/Gb per Run Pros Cons Biological Applications... 

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