Leukemia basophilic cells

Fig. 1. Time dependency of ADP-ribosylation of actin by C2 toxin in rot basophilic leukemia (RBL) cells. Rbl cells were incubated with C2 holotoxin (lOOng/ml C2I + 200ng/ml C2II) for the times indicated. Controls were incubation with the enzyme component C2I (lOOng/ml) alone, or with the binding component C2II (200ng/ml) alone, for 120 min. Another control (-) was not treated with toxin. After incubation, the medium was removed and cells were scraped off in the presence of lysis buffer (2 mM MgCb, 0.1 mM phenylmethylsulfonyl fluoride, 10 ig/ml leupeptin, 25 mM triethanolamine, pH 7.5), sonicated five times for 5sec on ice and centrifuged for 10 min at 1000 g. The supernatants were incubated with 1 [ig/ml C2I and 5nM [ P]NAD for 15 min at 30°C. The proteins were separated by 12.5% SDS-polyacrylamide gel electrophoresis and analyzed by phosphorimaging...

Hamasaki Y, Kobayashi I, et al. The Chinese herbal medicine, shinpi-to, inhibits IgE-mediated leukotriene synthesis in rat basophilic leukemia-2H3 cells. J Ethnopharmacol 1997 56 123-31. 

Fig. 13. Dissociation of DCT from IgE antibodies anchored on the surfaces of rat basophilic leukemia (RBL) cells. The rate of dissociation slows with time because of an increase in the number of free receptors. The effect is noticeable because pceN is not 1, and therefore kr is not constant. Reproduced from the Biophysical Journal, Goldstein et al. (1989), vol. 56, pp. 955-966, by copyright permission of the Biophysical Society.

Mast cell or basophil leukemia Carcinoid syndrome Thyroid carcinoma Pheochromocytoma... 

Hematopoiesis is defined as the development and maturation of blood cells and their precursors. In utero, hematopoiesis may occur in the liver, spleen, and bone marrow. However, after birth, it occurs exclusively in the bone marrow. All blood cells are generated from a common hematopoietic precursor, or stem cell. These stem cells are self-renewing and pluripotent and thus are able to commit to any one of the different lines of maturation that give rise to platelet-producing megakaryocytes, lymphoid, erythroid, and myeloid cells. The myeloid cell line produces monocytes, basophils, neutrophils, and eosinophils, whereas the lymphoid stem cell differentiates to form circulating B and T lymphocytes. In contrast to the ordered development of normal cells, the development of leukemia seems to represent an arrest in differentiation at an early phase in the continuum of stem cell to mature cell.1... 

An important characteristic of mammalian 15-LOX is its capacity to oxidize the esters of unsaturated acid in biological membranes and plasma lipoproteins without their hydrolysis to free acids. Jung et al. [19] found that human leukocyte 15-LOX oxidized phosphatidylcholine at carbon-15 of the AA moiety. Soybean and rabbit reticulocyte 15-LOXs were also active while human leukocyte 5-LOX, rat basophilic leukemia cell 5-LOX, and rabbit platelet 12-LOX were inactive. It was suggested that the oxygenation of phospholipid is a unique property of 15-LOX. However, Murray and Brash [20] showed that rabbit reticulocyte... 

Brock, T. G., McGish, R. W. and Peters-Golden, M. Translocation and leukotriene synthetic capacity of nuclear 5-lipoxygenase in rat basophilic leukemia cells and alveolar macrophages. /. Biol. Chem. 270 21652-21658,1995. 

R. M. Weis, K. Balakrishnan, B. A. Smith, and H. M. McConnell, Stimulation of fluorescence in a small contact region between rat basophil leukemia cells and planar lipid membrane targets by coherent evanescent radiation. J. Biol. Chem. 257, 6440-6445 (1982). 

Nomura et al. (2000) showed that hypoglycaemia-induced apoptosis of basophile leukemia ceUs involves the generation of cardiolipin hydroperoxide and the subsequent release of cytochrome c from mitochondria. (Nomura et al., 2000). The gaieration of cardiolipin hydroperoxide occurred in mitochondria of basophile leukaemia cells which had been induced to undergo apoptosis by exposure to hypoglycaemia with... 

LO activity is found in a variety of mammalian cells, most notably leukocytes, with the enzymes from neutrophils and the rat basophilic leukemia (RBL-1) cell line being the most studied several have been purified and cloned [5]. 5-LO is a cytosolic enzyme which requires calcium, ATP and additional uncharacterized cell components for optimal activity. Characterization and kinetic studies with 5-LO enzymes are often difficult, because of self-inactivation and complex cofactor requirements. 

Conklyn, M. J., Kadin, S. B., and Showell,H. J. (1990) Inhibition of IgE-mediated A-acetylglucosaminidase and serotonin release from rat basophilic leukemia cells (RBL-2H3) by Tenidap A novel anti- inflammatory agent. Int. Arch. Allerg. Appl. Immunol. 91, 369-373. 

Hammond, C. T. and P. G. Mahlberg. Phloroglucinol glucoside as a natural constituent of Cannabis sativa. Phytochemistry 1994 37(3) 755-756. Kataoka, M. and Y. Takagaki. Effect of the crude drugs (standards of natural drugs not in the J. P. XII) on beta-hexosaminidase release from rat basophilic leukemia (RBL-2H3) cells. Nat Med 1995 49(3) 346-349. 

DC086 Tanaka, Y., M. Kataoka, Y. Konishi, T. DC096 Nishimune, and Y. Takagaki. Effects of vegetable foods on beta-hexosaminidase release from rat basophilic leukemia cells (RBL-2H3). Jap J Toxicol EX2097 Environ Health 1992 38(5) 418-424. 

Histamine is formed by decarboxylation of the amino acid l -histidine, a reaction catalyzed in mammalian tissues by the enzyme histidine decarboxylase. Once formed, histamine is either stored or rapidly inactivated. Very little histamine is excreted unchanged. The major metabolic pathways involve conversion to /V-methylhistamine, methylimidazoleacetic acid, and imidazoleacetic acid (IAA). Certain neoplasms (systemic mastocytosis, urticaria pigmentosa, gastric carcinoid, and occasionally myelogenous leukemia) are associated with increased numbers of mast cells or basophils and with increased excretion of histamine and its metabolites. 

Chlorogenic and caffeic acids inhibit the activation of hyaluronidase, which is known as one of the enzymes involved in allergic effects [79], migration of cancer cells [80], inflammation [81, 82], and increased permeability of the vascular system. The superoxide anion radical scavenging activity of chlorogenic acids correlates linearly to the hyaluronidase-inhibitory activity. (3-Hexosaminidase release from rat basophilic leukemia cells induced by antigen has been inhibited by chlorogenic and caffeic acids. These compounds do not inhibit 13-hexosaminidase activity itself. 

Gao Z, Li BS, Day YJ, Linden J (2001) A3 adenosine receptor activation triggers phosphorylation of protein kinase B and protects rat basophilic leukemia 2H3 mast cells from apoptosis. Mol Pharmacol 59 76-82... 

Arachidonic acid released from membrane phospholipids or other sources is metabolized by the LO pathway to the smooth muscle contractile and vasoactive leukotrienes (LT), LTC4, and LTD4, as well as to the potent chemoattractant LTB4. These molecules are intimately involved in inflammation, asthma, and allergy, as well as in other multiple physiological and pathological processes. For example, cirsiliol (3, 4, 5-trihydroxy-6,7-dimethoxyflavone) proved to be a potent inhibitor of 5-LO (IC50, 0.1 pM) derived from basophilic leukemia cells and peritoneal polymorphonuclear leukocytes. 

Kurita, R., Hayashi, K., Horiuchi, T., Niwa, O., Maeyama, K., Tanizawa, K., Differential measurement with a microfluidic device for the highly selective continuous measurement of histamine released from rat basophilic leukemia cells (RBL-2H3). Labchip 2002, 2, 34-38. 

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