Leishmania tarentolae

Recently, another interaction was reported between an intracellular pathogen and the key regulator system of intracellular iron concentration, i.e. the degree of binding of the IRPs to IRE (see Chapter 7). In extracts from Leishmania tarentolae, a protein has been detected that binds specifically to the mammalian IRE. However, the exact nature and function of this protein is unclear up to now. In contrast to mammalian IRPs, the L. tarentolae IRE-binding activity was not induced by growth in iron-depleted medium (Meehan et ah, 2000). 

Editing of the cytochrome b message in the flagellated protozoan Leishmania tarentolae. Editing, which involves the insertion of 11 U residues, creates a translatable RNA where no translation was possible before. Structure shown for mRNA is after editing. 

Ouellette, M., Fase-Fowler, F. and Borst, P. (1990) The amplified H circle of methotrexate-resistant Leishmania tarentolae contains a novel P-glycoprotein gene. EMBOJ 9 1027-1033. 

Dey S, Papadopoulou B, Haimeur A et al. High level resistance in Leishmania tarentolae is mediated by an acdve extrusion system. Mol Biochem Parasitol 1994 67 49-57. 

Papadopoulou B, Roy G, Dey S et al. Gene disruption of the P ycoprotein related gene pgpa of Leishmania tarentolae. Biochem Biophys Res Commun 1996 224 772-778. 

Dey S, Ouellette M, Lightbody J et al. An ATP-dependent As(III)-g utathione transport system in membrane vesicles of Leishmania tarentolae. Proc Natl Acad Sci USA 1996 93 2192-2197. 

Kidane GZ, Ht es D, Simpson L. Sequence heterogeneity and anomalous dectrophoretic mobility of kinetoplast minidrde DNA from Leishmania tarentolae. Gene 1984 27 265-77. 

Sheline C, Ray DS. Specific discontinuities in Leishmania tarentolae minicircles map within universally conserved sequence blocks. Mol Biochem Parasitol 1989 37(2) 151-7. 

Yakovich AJ, Ragone FL, Alfonzo JD et al. Leishmania tarentolae Purification and characterizadon of tubulin and its suitability as a substrate for antileishmanial drug screening. Exp Parasitol 2006 in press. 

Manihot esculenta (cassava), MeHNL Protein purified by chromatography from the cyanogenic plant, N-terminal sequencing, peptide sequencing, degenerate primer design based on these sequences, cDNA library, gene identified Aliphatic, aromatic, and heteroaromatic a,p-unsaturated aldehydes and methyl ketones and aromatic ketones 29.5 (S) E. coli, P. pastoris, S. cerevisiae, Leishmania tarentolae [59,60[... 

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