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LC x UHPLC for Separations of Intact Proteins

One of the primary challenges facing the field of separation science is the analysis of the entire complement of proteins produced by an organism—a field of research known as proteomics. 2D gel electrophoresis remains the gold standard in protein separations, with the ability to resolve as many as 5000 proteins in a single gel [Pg.191]

FIGURE 8.10 Basic configuration of instrumentation used for off-line AEX x UHP-RPLC. [Pg.192]


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Intact protein

LC Separation

Separation of proteins

Separator Protein

UHPLC

X-protein

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