Mixed-associative lateral interactions

Analytes from class N neither self-associate nor participate in the mixed hydrogen bonds. Consequently, they cannot participate in lateral interactions of any kind, either. 

Analytes from classes A and B cannot self-associate through the hydrogen bonds and because of this, they cannot participate in the self-associative lateral interactions. However, they can participate in the mixed hydrogen bonds and take part in the mixed lateral interactions. For example, analyte A can laterally interact either with analyte B or analyte AB. In a similar way, analyte B can either interact with analyte A or analyte AB. 

Mixed lateral interactions can occur in the case of the following pairs of molecules A. .. B, AB. .. A, AB. .. B, and ABj. .. ABj. Their appearance is even more importunate than that of the self-associative lateral interactions. With the self-asso-ciative lateral interactions alone, tailing of chromatographic bands lowers separative performance of a given chromatographic system, whereas with the mixed lateral... 

Now we are going to demonstrate mixed lateral interactions for the two different AB. .. B systems (the first composed of acid and ketone and the second composed of alcohol and ketone) and to discnss their impact on separation performance when using mild chromatographic cotrditiotrs (i.e., the low-active adsorbent and the low-polar eluent). As the binary test systems, we selected the 2-phenylbutyric acid-benzophenone mixture of analytes (i.e., acid and ketone) and the 5-phenyl-l-pentanol-benzophenone mixture of analytes (i.e., alcohol and ketone). Mixed associative dimers composed of the two pairs of analytes are schematically given in Figure 2.17. 

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