Laser confocal microscopy -differential

Fig. 10.11 Adsorption of 3LNPs with PKH26 on SKOV-3 cell membrane at pH 7.4 (a, b) and 6.0 (c, d) at 4°C observed with confocal scanning laser fluorescence microscopy. Differential interference contrast (a, c) and red fluorescence channel (b, d)...

LCM-DIM laser confocal microscopy combined with differential... 

CD Circular dichroism Die Differential interference contrast DLS Dynamic light scattering FTIR Fourier-transform infrared LSCM Laser scanning confocal microscopy LCST Lower critical solution temperature Me Methyl... 

Fig. 1. Wound healing and inflammation in Drosophila embryos, (a) A laser ablation wound to a moesinGFP expressing Drosophila embryo will, by a purse-string mechanism, (b) seal shut over a period of a few hours, (c) This same laser wound will also recmit hemo-cytes that, by driving GFP specifically in these cells, can be imaged by widefield microscopy. (d) Alternatively, the wound site can be imaged by Differential Interference Contrast microscopy to give a general idea of wound size (dotted line) and hemocyte presence ( ). (e) The insertion of a heparin bead into the wound site will also lead to the recruitment of hemocytes that will surround and encapsulate the bead, (f) A hemocyte undergoing developmental dispersal viewed by confocal microscopy.

More advanced (and expensive) microscopy techniques are differential interference contrast microscopy and laser scanning confocal microscopy. The latter can provide precise optical sectioning so that three-dimensional images of stmctures can be recorded. 

Rocheleau S., Greer C. W., Lawrence J. R., Cantin C., Laramee L., and Guiot S. R. (1999) Differentiation of Methanosaeta concilii and Methanosarcina barkeri in anaerobic mesophi-Uc granular sludge by fluorescent in situ hybridization and confocal scanning laser microscopy. Appl Environ. Microbiol 65, 2222-2229. 

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