Laminin peptide sequence

Further improvements have been achieved using thiolated and non-thiolated hydro)g apatite-coated crab-tendon chitosan (t-chitosan/HAP-SH and t-chitosan/HAP, respectively) tubes, with and without a conjugated laminin peptide sequence (YIGSR). Laminin-1 is considered to be one of the most important cell adhesion molecules, and a pentapeptide sequence (YIGSR) located at its B1 domain is believed to enhance Schwann-cell migration and attachment, as well as neural outgrowth. 

Fig. 3. Schematic representation (not to scale) of the amino acid sequence of the A-S-B2 isomer of laminin. Laminin is composed of one long arm (A) and two short arms (S and B2) arranged in a cruciform configuration 70 nm long and 70 nm wide. The arginine-glycine-aspartic acid adhesive peptide ( ) starts at peptide sequence 2534 in chain A. (The schematic was redrawn using information obtained from the following references Ayad et al, 1994 Mathews and van Holde, 1990 and Darnell et al, 1990.)...

Stauffer, W., and X.T. Cui. 2006. Polypyrrole doped aith 2 peptide sequences from laminin. Biomaterials 27 2405. 

The second approach includes native long chain of ECM proteins as well as short peptide sequences derived from intact ECM proteins that are able to interact specifically with cell receptors and to confer appropriate functions to the biomaterials [102]. The most studied class of cell-adhesive molecules comprises ECM-soluble serum proteins, including fibronectin (FN), vitronectin (VN), collagen families, thrombospondin, osteopontin, fibrinogen, von Willebrand factor and laminin (LN) [87]. 

Synthetic laminin-1 A10 and B160 peptide sequences were coupled to poly (L-lysine) coated glass or fibrin gels. In order to investigate a broad range of peptide surface concentrations in a single test specimen, a gradient in the peptide concentration on the polymer surfaces was prepared in one direction. The... 

---