Lambda ZAP

Lambda ZAP II Library, products of Stratagene, Cat. no. 937010 and 936204, respectively. rfBelow the detectable level. 

Short JM, Fernandez JM, Sorge JA, Huse WD Lambda ZAP A bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res 1988 16 7583-7600. 

Elegant and efficient subtractive hybridization procedures have been developed from the original procedure described by Sive and St John (1988) and Duguid et al. (1988) in which driver cDNA is photobi-otinylated, allowed to react with the (-I-) DNA and the hybrids then eliminated with streptavidin agarose, Schweinfest et al. (1990) used the lambda ZAP II phage vector system to produce by in vivo excision a large amount of DNA for biotinylation. Recent methods take advantage of the property of biotinylated nucleic acids... 

Kohler, S. W., Provost, G. S., Fieck, A., Kretz, P. L., Bullock, W. O., Putman, D. L., Sorge, J. A., and Short, J. M. (1991a). Analysis of spontaneous and induced mutations in transgenic mice using a lambda ZAP/lacl shuttle vector. Environ Mol Mutagen 18, 316-321. 

Fig. 1. Differential screening of a human cDNA library with subtracted PolyA cDNA probes. A human cDNA library is prepared using the lambda cloning vector Lambda ZAP (Stratagene). Two replica filters are produced from a single petri dish containing approximately 2,000 recombinant clones. One filter is hybridized with a probe enriched for sequences expressed in unilineage cells (U-M), and the other hybridized with a probe enriched for sequences expressed in multilineage cells (U-M). The arrows indicate clones that were hybridized strongly with one probe and not the other.

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