Lambda DNA

Murialdo, H., 1991. Bacteriophage lambda DNA maturation and packaging. Annual Review of Biochemistry 60 125-153. Review of the biochemi.stry of packaging DNA into A phage heads. 

A somewhat similar approach has been nsed for the formation of nanosize wires stretching between gold electrodes [34]. Lambda-DNA was positioned between two electrodes, with immobilized oligonncleotides complementary to lambda-DNA sticky ends. Silver (Ag ) ions were deposited on the stretched DNA bridges, followed by rednction of absorbed ions to metallic silver with hydroquinone. The resulting silver clusters formed on DNA strands were found to be 100 nm in diameter and were capable of condncting the electric current. 

Figure 5.26 Integration of lambda DNA into the host. Integration always occurs at a specific site on the host DNA, involving a specific attachment site (att) on the phage. Some of the host genes near the attachment site are given. A sitespecific enzyme (integrase) is involved, and specific pairing of the complementary ends results in integration of phage DNA.

Replication Replication of lambda DNA occurs in two distinct fashions during different parts of the phage production cycle. Initially, liberation of lambda DNA from the host results in replication of a circular DNA, but subsequently linear concatamers are formed, which replicate in a different way. Replication is initiated at a site close to gene 0 and from there proceeds in opposite directions (bidirectional... 

Marx, K.A. and Ruben, G.C. (1986) A study of fX-174 DNA torus and lambda DNA torus tertiary structure and the implication for DNA self-assembly. J. Biomol. Struct. Dyn., 4, 23-39. 

Liberek, K., Georgopoulos, C., and Zylicz, M. (1988). Role of the Escherichia coli DnaK and DnaJ heat shock proteins in the initiation of bacteriophage lambda DNA replication. Proc. Natl. Acad. Sci. U.S.A. 85, 6632—6636. 

Mensa-Wilmot, K., Seaby, R., Alfano, C., Wold, M. C Gomes, B., and McMacken, R. (1989). Reconstitution of a nine-protein system that initiates bacteriophage lambda DNA replication, y. Biol. Chem. 2M, 2853-2861. 

Yochem, J., Uchida, H., Sunshine, M., Saito, H., Georgopoulos, C., and Feiss, M. (1978). Genetic analysis of two genes, dnaj and dnaK, necessary ior Escherichia coli and bacteriophage lambda DNA replication. Mol. Gen. Genet. 164, 9-14. 

Zylicz, M., Ang, D., Liberek, K., Yamamoto, T., and Georgopoulos, C. (1988). Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins. Biochim. Biophys. Acta 951, 344-350. 

Fig. 3.50 A 2.5 x 2.5 im2 AFM image of lambda DNA on APS-modified mica acquired in CM-AFM in Tris buffer. This image was collected in normal contact mode, with loading force of 1 nN, using a Park sharpened tip (cantilever force constant of 0.1 N/m). Reproduced with permission from [106]. Copyright 1996. American Chemical Society...

Prepare a lambda-DNA or T2-DNA ladder to be used as size markers. The method of Vollrath and Davis (1987) and Sambrook et al. (1989) using lambda-DNA yields a nice set of concatemers ... 

Gunther, E. J., Murray, N. E., and Glazer, P. M. (1993). High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E. coli lysogen. Nucleic Acids Res 21, 3903-3904. 

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