LacZ bacterial gene

Detection of gene mutations in the lacZ bacterial reporter gene. 

A. Kruger, V. Schirrmacher, R. Khokha, The bacterial lacZ gene An important tool for metastasis research and evaluation if new cancer therapies. Cancer Metastasis Rev. 17 (1999) 285-294. 

There are several methods to identify bacterial colonies harboring recombinant plasmid. One of the more commonly used methods is referred to as a-complementa-tion. The pUC series of plasmids contains the coding sequence for the first 146 amino acids of the amino terminus of the (S-galactosidase gene (lacZ). Within this... 

Kobayashi, K., Nakanishi, H., Inada, K., Fujimitsu, Y., Yamachika, T., Shi-rai, T. and Tatematsu, M. (1996). Growth characteristics in the initial stage of micrometastasis formation by bacterial LacZ gene-tagged rat prostatic adenocarcinoma cells. Jpn. J. Cancer Res. 87, 1227-1234... 

Lin, W. C., Pretlow, T. P., Pretlow, T. G. 2d and Culp, L. A. (1990a) Development of micrometastases earliest events detected with bacterial lacZ gene-tagged tumor cells. J. Natl. Cancer Inst. 82,1497-1503. 

Andersen JK, Garber DA, Meaney CA, Breakefield XO (1992) Gene transfer into mammalian central nervous system using herpes virus vectors Extended expression of bacterial lacZ in neurons using the neuron-specific enolase promoter. Hum Gene Ther 3 487-499. 

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