Laboratory Scale Production of Lactic Acid

Fermentation is the most common approach used for the production of lactic acid. The method that is outlined here (Ohara et al., 2003) can be utilized to synthesize lactic acid in lactate form for prepolymer lactic acid production  

5 L of culture medium is prepared, which consists of 500 g glucose, 100 g yeast extract and 100 g polypeptone. The medium is sterilized using an autoclave and finally a microbe species, from one of the flowing genera is implanted Lactobacillus, Streptococcus, Rhizopus, Bacillus or Leuconostoc. 

1 Method for Synthesis of Lactic Acid in Lactate Form—cont d 

The mixture is cultured at a temperature of 37°C, with the pH maintained at 7.0 using 6N ammonia-water. The culture takes 15 hours to complete. 

The culture is concentrated using 1000 g of ethanol and refluxed for 3 hours at between 90 and 100°C in a condenser to obtain ethyl lactate. 

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