Labile zinc

Haug FM (1967) Electron microscopical localization of the zinc in hippocampal mossy fibre synapses by a modified sulfide silver procedure. Histochemie 8 355-368 Hebert LE, Scherr PA, Bienias JL, Bennett DA, Evans DA (2003) Alzheimer disease in the US population prevalence estimates using the 2000 census. Arch Neurol 60 1119-1122 Ho LH, Ratnaike RN, Zalewski PD (2000) Involvement of intracellular labile zinc in suppression of DEVD-caspase activity in human neuroblastoma cells. Biochem Biophys Res Commun 268 148-154... 

Zalewski PD, Forbes IJ, Seamark RF, Boilinghaus R, Betts WH, Lincoln SF. Wtird AD (1994) Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revetiled by a specific chemical probe, Zinquin. Chem Biol 1 153-161... 

Labile Zinc Zinc is an essential mineral for most organisms. Zn is either labile or fixed. 

G., and Rink, L., Flow cytometric measnrement of labile zinc in peripheral blood mononuclear cells, Analyt. Biochem. 352, 222-230, 2006. 

Some of the most intriguing differences between the met-allomes of different cell types occur in subcellular organelles or vesicles. Eukaryotic cells in particular have carefully compartmentalized essential transition metals for specific biological purposes. The mitochondria and the chloroplast both contain high levels of metalloproteins relative to the cytoplasm and may have distinct metal quotas. Mammahan cerebrocortical neurons possess zinc-filled vesicles with labile zinc pools that approach... 

We also used CD to ascertain the effects on structure and stability of the ZDD domain upon removal of the labile-bound RING finger zinc ion. In this case, the CD spectrum shows a 36% loss in molar ellipticity at 204 nm as compared to the native domain, indicating partial unfolding upon release of the metal ion from the labile site (shown in Figure 2A). These observations support the conclusion that the labile zinc plays an important role in the determination and stabilization of the local secondary structure in the RING finger subdomain. 

Zinc complexation was evaluated by ligand exchange with ethylenediami-netetraacetic acid (EDTA) and anodic stripping voltammetry of the labile zinc (27). Labile and nonlabile Zn species were distinguished by voltammetry. The labile Zn complexes were evaluated by competition with added EDTA. The Zn-EDTA complex is voltammetrically nonlabile. The decrease in labile Zn concentration was thus measured as a function of added EDTA, and the concentration of [Zn2+] was calculated from the [Zn-EDTA] formed and the equilibrium with free EDTA. 

Courchesne, F., Kruyts, N., and Legrand, P. (2006). Labile zinc concentration and free copper ion activity in the rhizosphere of forest soils. Environ. Toxicol. Chem. 2S, 635-642. 

Overall, [([12]aneN3)Zn(0H2)](C104)2 exhibits pH-dependent catalytic behavior for the hydration of C02 and dehydration of HCO. The rate-determining step of the C02 hydration reaction is the uptake of C02 by the zinc hydroxide complex. The rate-determining step in bicarbonate dehydration is substitution of the labile zinc-bound water molecule by the bicarbonate anion. The overall catalytic mechanism for both reactions is shown in Scheme 4. In considering this mechanism, it is... 

Evans, G.W., Johnson, P.E., Brushmiller, J.G. and Ames, R.W. (1979). Detection of Labile Zinc-Binding Ligands in Biological Fluids by Modified Gei Filtration Chromatography. Anal. Chem., 51, 839. 

In acetate buflFer, all of the labile zinc undergoes exchange with a single rate constant, the absolute value of which depends upon enzyme concentration (18) (Figure llA). In contrast, in phosphate buflFer, het-... 

Kim J, Kim TY, Hwang JJ, Lee JY, Shin JH, Gwag BJ, Koh JY (2009) Accumulation of labile zinc in neurons and astrocytes in the spinal cords of G93A SOD-1 transgenic mice. Neuiobiol Dis 34 221-229... 

For the labile zinc determination it is preferable to select a more positive adsorption potential. In this condition the sensitivity for zinc is still about ten times greater than that for nickel and cobalt, so these metals do not interfere unless their concentrations are much greater than usual. 

Truong-Tran, A. Q. Ruffin R. E. Zalewski, P D. Visualizafion of labile zinc and ifs role in apopfosis of primary airway epifhelial cells and cell lines. Am. J. Physiol. 2000, 279, L1172-L1183. 

Zalewski, P. D. Millard, S. H. Forbes, I. J. Kapaniris, O. Slavotinek, A. Betts, W. H. Ward, A. D. Lincoln, S. F. Mahadevan, I. Video image analysis of labile zinc in viable pancreatic islet cells using a specific fluorescent probe for zinc. J. Histochem. Cytochem. 1994, 42, 877-884. 

Sediment conditions for the adsorbed fiaction of zinc and copper were determined from Terrace Reservoir data (8). The data showed that the operationally defined labile zinc and copper in the sediments were about 49.2 5.26 % and 23.1 3.62 % of the total sediment burden ctf these metals. The iron oxide fiaction for iron was approximately 21.8 7.10 % of the total iron content The conditions were used to descrite the partitioning of copper and zinc to the sediments as reflected in double l er sorption model used in WASP4/META4. 

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