Kinetic methods chips used

Figure 7.4 Reactions in microfluidic chips coupled with a MS system, (a) Photograph of the two-inlet chip used to study reaction kinetics, (b) Reaction profile (markers) of the on-chip derivatization of different compounds with NBDPZ and corresponding fits to a second-order kinetics model (lines) [51]. Adapted with permission from Brivio, M., Liesener, A., Oosterbroek, R.E., Verboom, W., Karst, U., van den Berg, A., Reinhoudt, D.N. (2005) Chip-based On-line Nanospray MS Method Enabling Study of the Kinetics of Isocyanate Derivatization Reactions. Anal. Chem. 77 6852-6856. Copyright (2005) American Chemical Society...

Some methacrylate chips built by combined use of VCarve and MACHS, (a) Chip on valve mounted atop the selection valve, (b) Chip designed for cross-injection analysis, (c) Chip especially used in kinetic methods. (For color version of this figure, the reader is referred to the online version of this book.)... 

Purification. Benson, McBee, and Rand, in submitting an Organic Syntheses procedure for the preparation of N-iodosuccmimide, purify dioxane only by use of sodium chips and distillation (reference to Fieser"). Checkers B. C. McKusick and T. J. Kealy used a newly opened bottle of spectroquality Reagent (MCB). In purifying the solvent for a kinetic study Burstein and Ringold state that they applied the method of Fieser and then stored the solvent over a Linde molecular sieve for at least a week. Aetually Fieser cited two methods from the literature, the first of which is designed to eliminate water and glycol acetal by hydrolysis to acetaldehyde. The methods are as follows ... 

MALDI-TOF-MS has been demonstrated as a useful tool in pre-steady state kinetic research by Houston et al., who combined it off-line with quench-flow methods to follow the appearance of a protein tyrosine phosphatase (PTPase) reaction intermediate.12 Houston et al. were able to measure rate constants up to 30 s 1, with k2/k3 ratios up to approximately 15. The device described in this chapter extends this technique to measure rate constants approximately 5 times greater, with k2/k3 ratios approximately twice previously measurable values. MALDI-TOF-MS is typically conducted on a centimeter-scale conducting plate the digital microfluidic system employed is a square plate approximately 2 cm on each side, with 16 experimental units per chip, which can be placed directly inside a standard MALDI-TOF-MS plate that has been machined appropriately. By grounding the exposed wires on the otherwise insulated chip, charging is negligible. 

Kinetic sample injection is a method which employs electroosmotic flow to load and separate the samples for chemical and biomedical analysis in many bio- or chemical Lab-on-a-Chip. It is one of the injection techniques used for the microfluidic injector within microfluidic devices. The typical volume delivered by kinetic injection is about htmdreds of picoliters. 

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